pRSFDuet-1

pRSFDuet-1

pRSFDuet1载体基本信息

出品公司:	Ybscience
别名:	pRSFDuet1, pRSFDuet 1
质粒类型:	大肠杆菌蛋白双表达载体，有两个多克隆位点
表达水平:	高
克隆方法:	多克隆位点，限制性内切酶
载体大小:	3829 bp
5' 测序引物:	T7
5' 测序引物序列:	T7: 5'-TAATACGACTCACTATAGGG-3';
3' 测序引物:	T7t
3' 测序引物序列:	T7t: 5'-TGCTAGTTATTGCTCAGCGG-3'
载体标签:	N-His, C-S
载体抗性:	Kanamycin
备注:	pRSFDuet-1 carries the RSF1030 replicon, lacI gene and kanamycin resistance gene.
稳定性:	瞬时表达 Transient
组成型:	组成型 Constitutive
病毒/非病毒:	非病毒

pRSFDuet1载体质粒图谱和多克隆位点信息

pRSFDuet1载体简介

pRSFDuet-1 is designed for the coexpression of two target ORFs. The vector contains two multiple cloning sites (MCS), each of which is preceded by a T7lac promoter and ribosome binding site (rbs). The vector also carries the RSF1030-derived RSF replicon, lacI gene and kanamycin resistance gene (KnR). This vector can be used in combination with pACYCDuet™-1 (Cat. No. 71147-3), pCDFDuet™-1 (Cat. Np. 71340-3), and pETDuet™-1 (Cat. No. 71146-3) in an appropriate host strain for the coexpression of 4 to 8 target proteins. ORFs inserted into MCS1 can be sequenced using the ACYCDuetUP1 Primer (Cat. No. 71178-3) and DuetDOWN1 Primer (Cat. No. 71179-3). ORFs inserted into MCS2 can be sequenced using the DuetUP2 Primer (Cat. No. 71180-3) and T7 Terminator Primer (Cat. No. 69337-3). Unique sites are shown on the circle map.

pRSFDuet1载体序列

LOCUS       pRSFDuet-1    3829 bp     DNA  Circular   SYN
DEFINITION  pRSFDuet-1
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
COMMENT     This file is created by Vector NTI
            http://www.biofeng.com/
COMMENT     ORIGDB|GenBank
COMMENT     VNTAUTHORNAME|biofeng.com|
COMMENT     VNTOAUTHORAD1|Novagen Inc.|
FEATURES             Location/Qualifiers
     source          1..3829
                     /organism="pRSFDuet-1"
                     /mol_type="other DNA"
     misc_feature    2..29
                     /label="lacO"
     promoter        214..232
                     /label="T7_promoter"
     misc_feature    232..259
                     /label="lacO"
     misc_feature    complement(448..466)
                     /label="T7_Terminal_primer"
     gene            complement(739..1554)
                     /label="KanR2"
                     /gene="KanR2"
     CDS             complement(739..1554)
                     /label="ORF frame 2"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     promoter        complement(1596..1624)
                     /label="AmpR_promoter"
                     /translation="MSHIQRETSCSRPRLNSNMDADLYGYKWARDNVGQSGATIYRLY
                     GKPDAPELFLKHGKGSVANDVTDEMVRLNWLTEFMPLPTIKHFIRTPDDAWLLTTAIP
                     GKTAFQVLEEYPDSGENIVDALAVFLRRLHSIPVCNCPFNSDRVFRLAQAQSRMNNGL
                     VDASDFDDERNGWPVEQVWKEMHKLLPFSPDSVVTHGDFSLDNLIFDEGKLIGCIDVG
                     RVGIADRYQDLAILWNCLGEFSPSLQKRLFQKYGIDNPDMNKLQFHLMLDEFF*"
     CDS             complement(2607..3566)
                     /label="ORF frame 3"
                     /translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
                     RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
                     PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
                     HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
                     SGLRVGADISVVGYDDTEDSSCYIPPLTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
                     LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
     CDS             complement(3439..158)
                     /label="ORF frame 3"
                     /translation="MRPQACRPAGAPSSNSDPGCGDDGDGCCPWYISLLKLNKIISTG
                     ELLSAHNSPIVSRINFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECET
                     SNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTV
                     VADWRCHLQSGPARAVANCRGD*"
     promoter        3813..2
                     /label="T7_promoter"
                     /translation="MRPQACRPAGAPSSNSDPGCGDDGDGCCPWYISLLKLNKIISTG
                     ELLSAHNSPIVSRINFLMQESHKGERRDPGHHRMAQNLSRYGMIAPGRESIQGGECET
                     SNVIRCRRVCRCLLSDRFPRGEPGQPRFCENAGKSGSGDGGAELHSQPRGTTTGGQTV
                     VADWRCHLQSGPARAVANCRGD*"
ORIGIN
    1 ggggaattgt gagcggataa caattcccct gtagaaataa ttttgtttaa ctttaataag
   61 gagatatacc atgggcagca gccatcacca tcatcaccac agccaggatc cgaattcgag
  121 ctcggcgcgc ctgcaggtcg acaagcttgc ggccgcataa tgcttaagtc gaacagaaag
  181 taatcgtatt gtacacggcc gcataatcga aattaatacg actcactata ggggaattgt
  241 gagcggataa caattcccca tcttagtata ttagttaagt ataagaagga gatatacata
  301 tggcagatct caattggata tcggccggcc acgcgatcgc tgacgtcggt accctcgagt
  361 ctggtaaaga aaccgctgct gcgaaatttg aacgccagca catggactcg tctactagcg
  421 cagcttaatt aacctaggct gctgccaccg ctgagcaata actagcataa ccccttgggg
  481 cctctaaacg ggtcttgagg ggttttttgc tgaaacctca ggcatttgag aagcacacgg
  541 tcacactgct tccggtagtc aataaaccgg taaaccagca atagacataa gcggctattt
  601 aacgaccctg ccctgaaccg acgacaagct gacgaccggg tctccgcaag tggcactttt
  661 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
  721 ccgctcatga attaattctt agaaaaactc atcgagcatc aaatgaaact gcaatttatt
  781 catatcagga ttatcaatac catatttttg aaaaagccgt ttctgtaatg aaggagaaaa
  841 ctcaccgagg cagttccata ggatggcaag atcctggtat cggtctgcga ttccgactcg
  901 tccaacatca atacaaccta ttaatttccc ctcgtcaaaa ataaggttat caagtgagaa
  961 atcaccatga gtgacgactg aatccggtga gaatggcaaa agtttatgca tttctttcca
 1021 gacttgttca acaggccagc cattacgctc gtcatcaaaa tcactcgcat caaccaaacc
 1081 gttattcatt cgtgattgcg cctgagcgag acgaaatacg cggtcgctgt taaaaggaca
 1141 attacaaaca ggaatcgaat gcaaccggcg caggaacact gccagcgcat caacaatatt
 1201 ttcacctgaa tcaggatatt cttctaatac ctggaatgct gttttcccgg ggatcgcagt
 1261 ggtgagtaac catgcatcat caggagtacg gataaaatgc ttgatggtcg gaagaggcat
 1321 aaattccgtc agccagttta gtctgaccat ctcatctgta acatcattgg caacgctacc
 1381 tttgccatgt ttcagaaaca actctggcgc atcgggcttc ccatacaatc gatagattgt
 1441 cgcacctgat tgcccgacat tatcgcgagc ccatttatac ccatataaat cagcatccat
 1501 gttggaattt aatcgcggcc tagagcaaga cgtttcccgt tgaatatggc tcatactctt
 1561 cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
 1621 tgaatgtatt tagaaaaata aacaaatagg catgcagcgc tcttccgctt cctcgctcac
 1681 tgactcgcta cgctcggtcg ttcgactgcg gcgagcggtg tcagctcact caaaagcggt
 1741 aatacggtta tccacagaat caggggataa agccggaaag aacatgtgag caaaaagcaa
 1801 agcaccggaa gaagccaacg ccgcaggcgt ttttccatag gctccgcccc cctgacgagc
 1861 atcacaaaaa tcgacgctca agccagaggt ggcgaaaccc gacaggacta taaagatacc
 1921 aggcgtttcc ccctggaagc tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg
 1981 gatacctgtc cgcctttctc ccttcgggaa gcgtggcgct ttctcatagc tcacgctgtt
 2041 ggtatctcag ttcggtgtag gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg
 2101 ttcagcccga ccgctgcgcc ttatccggta actatcgtct tgagtccaac ccggtaagac
 2161 acgacttatc gccactggca gcagccattg gtaactgatt tagaggactt tgtcttgaag
 2221 ttatgcacct gttaaggcta aactgaaaga acagattttg gtgagtgcgg tcctccaacc
 2281 cacttacctt ggttcaaaga gttggtagct cagcgaacct tgagaaaacc accgttggta
 2341 gcggtggttt ttctttattt atgagatgat gaatcaatcg gtctatcaag tcaacgaaca
 2401 gctattccgt tactctagat ttcagtgcaa tttatctctt caaatgtagc acctgaagtc
 2461 agccccatac gatataagtt gtaattctca tgttagtcat gccccgcgcc caccggaagg
 2521 agctgactgg gttgaaggct ctcaagggca tcggtcgaga tcccggtgcc taatgagtga
 2581 gctaacttac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga aacctgtcgt
 2641 gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt attgggcgcc
 2701 agggtggttt ttcttttcac cagtgagacg ggcaacagct gattgccctt caccgcctgg
 2761 ccctgagaga gttgcagcaa gcggtccacg ctggtttgcc ccagcaggcg aaaatcctgt
 2821 ttgatggtgg ttaacggcgg gatataacat gagctgtctt cggtatcgtc gtatcccact
 2881 accgagatgt ccgcaccaac gcgcagcccg gactcggtaa tggcgcgcat tgcgcccagc
 2941 gccatctgat cgttggcaac cagcatcgca gtgggaacga tgccctcatt cagcatttgc
 3001 atggtttgtt gaaaaccgga catggcactc cagtcgcctt cccgttccgc tatcggctga
 3061 atttgattgc gagtgagata tttatgccag ccagccagac gcagacgcgc cgagacagaa
 3121 cttaatgggc ccgctaacag cgcgatttgc tggtgaccca atgcgaccag atgctccacg
 3181 cccagtcgcg taccgtcttc atgggagaaa ataatactgt tgatgggtgt ctggtcagag
 3241 acatcaagaa ataacgccgg aacattagtg caggcagctt ccacagcaat ggcatcctgg
 3301 tcatccagcg gatagttaat gatcagccca ctgacgcgtt gcgcgagaag attgtgcacc
 3361 gccgctttac aggcttcgac gccgcttcgt tctaccatcg acaccaccac gctggcaccc
 3421 agttgatcgg cgcgagattt aatcgccgcg acaatttgcg acggcgcgtg cagggccaga
 3481 ctggaggtgg caacgccaat cagcaacgac tgtttgcccg ccagttgttg tgccacgcgg
 3541 ttgggaatgt aattcagctc cgccatcgcc gcttccactt tttcccgcgt tttcgcagaa
 3601 acgtggctgg cctggttcac cacgcgggaa acggtctgat aagagacacc ggcatactct
 3661 gcgacatcgt ataacgttac tggtttcaca ttcaccaccc tgaattgact ctcttccggg
 3721 cgctatcatg ccataccgcg aaaggttttg cgccattcga tggtgtccgg gatctcgacg
 3781 ctctccctta tgcgactcct gcattaggaa attaatacga ctcactata