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文献和实验Protocol for Trichloroacetic Acid (TCA) Precipitation
Introduction The efficiency of nucleotide incorporation in DNA/RNA polymerization reactions (e.g. transcription, reverse transcription, and DNA replication) can be determined by trichloroacetic acid (TCA) precipitation. TCA
Phosphoamino acid analysis:Mark Kamps method
Phosphoamino acid analysis:Mark Kamps's method 1. Label your protein with 32Pi. Then subject the protein to SDS polyacrylamide gel electrophoresis and transfer your gel-fractionated protein to Immobilon-P .
How to Make Simple Solutions and Dilutions
Chemical FW = 194.3 g/mole; to make 0.15 M solution use 194.3 g/mole * 0.15 moles/L = 29.145 g/L Example 2: To prepare a specific volume of a specific molar solution from a dry
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