- ¥800 - 1200
- Ybscience
- 中国/美国
- YB-0134
- 2025年07月16日
13 年金牌会员
企业认证
万千商家帮你免费找货
0 人在求购买到急需产品
- 详细信息
- 文献和实验
- 技术资料
- 保存条件：
-20℃
- 保质期：
6个月
- 英文名：
pSE380
- 库存：
大量
- 供应商：
钰博生物
- 规格：
1ug/5ug
pSE380
pSE380载体基本信息
| 出品公司: | Ybscience |
|---|---|
| 载体名称: | pSE380 |
| 质粒类型: | 大肠杆菌表达载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 启动子: | trc |
| 克隆方法: | 多克隆位点，限制性内切酶 |
| 载体大小: | 4476 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | 无标签 |
| 载体抗性: | 氨苄青霉素（Ampicillin） |
| 筛选标记: | -- |
| 备注: | E.coli NM522, E.coli INValphaF' 相关载体: pTrc99A, pSE280, pSE420 |
| 稳定性: | -- |
| 组成型: | -- |
| 病毒/非病毒: | -- |
pSE380载体质粒图谱和多克隆位点信息
pSE380载体简介
pSE380载体序列
LOCUS pSE380 4476 bp DNA SYN
DEFINITION pSE380
ACCESSION
KEYWORDS
SOURCE
ORGANISM other sequences; artificial sequences; vectors.
FEATURES Location/Qualifiers
source 1..4476
/organism="pSE380"
/mol_type="other DNA"
promoter 202..275
/label="trc_promoter"
misc_feature 244..266
/label="M13_pUC_rev_primer"
misc_feature complement(659..676)
/label="pTrcHis_rev_primer"
misc_feature complement(659..676)
/label="pBAD_rev_primer"
terminator 709..866
/label="rrnB_terminator"
terminator 832..875
/label="rrnB_T1_terminator"
terminator 1007..1034
/label="rrnB_T2_terminator"
promoter 1076..1104
/label="AmpR_promoter"
gene 1146..2006
/label="Ampicillin"
/gene="Ampicillin"
CDS 1146..2006
/label="ORF frame 3"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
rep_origin 2161..2780
/label="pBR322_origin"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 3177..3199
/label="pGEX_3_primer"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
misc_feature 3346..4437
/label="lacI"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
DRWEPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
EIGASLIKHW*"
CDS 3478..4437
/label="ORF frame 1"
/translation="MAELNYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKS
RADQLGASVVVSMVERSGVEACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNV
PALFLDVSDQTPINSIIFSHEDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGW
HKYLTRNQIQPIAEREGDWSAMSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITE
SGLRVGADISVVGYDDTEDSSCYIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQ
LLPVSLVKRKTTLAPNTQTASPRALADSLMQLARQVSRLESGQ*"
ORIGIN
1 aattctcatg tttgacagct tatcatcgac tgcacggtgc accaatgctt ctggcgtcag
61 gcagccatcg gaagctgtgg tatggctgtg caggtcgtaa atcactgcat aattcgtgtc
121 gctcaaggcg cactcccgtt ctggataatg ttttttgcgc cgacatcata acggttctgg
181 caaatattct gaaatgagct gttgacaatt aatcatccgg ctcgtataat gtgtggaatt
241 gtgagcggat aacaatttca cacaggaaac agaccatggc tggtgaccac gtcgtggaat
301 gccttcgaat tcagcacctg cacatgggac gtcgacctga ggtaattata acccgggccc
361 tatatatgga tccaattgca atgatcatca tgacagatct gcgcgcgatc gatatcagcg
421 ctttaaattt gcgcatgcta gctatagttc tagaggtacc ggttgttaac gttagccggc
481 tacgtatact ccggaatatt aataggccta ggatgcatat ggcggccgcc tgcagctggc
541 gccatcgata cgcgtacgtc gcgaccgcgg acatgtacag agctcgagaa gtactagtgg
601 ccacgtgggc cgtgcacctt aagcttggct gttttggcgg atgagagaag attttcagcc
661 tgatacagat taaatcagaa cgcagaagcg gtctgataaa acagaatttg cctggcggca
721 gtagcgcggt ggtcccacct gaccccatgc cgaactcaga agtgaaacgc cgtagcgccg
781 atggtagtgt ggggtctccc catgcgagag tagggaactg ccaggcatca aataaaacga
841 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
901 ctgagtagga caaatccgcc gggagcggat ttgaacgttg cgaagcaacg gcccggaggg
961 tggcgggcag gacgcccgcc ataaactgcc aggcatcaaa ttaagcagaa ggccatcctg
1021 acggatggcc tttttgcgtt tctacaaact ctttttgttt atttttctaa atacattcaa
1081 atatgtatcc gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga
1141 agagtatgag tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc
1201 ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg
1261 gtgcacgagt gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc
1321 gccccgaaga acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat
1381 tatcccgtgt tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg
1441 acttggttga gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag
1501 aattatgcag tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa
1561 cgatcggagg accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc
1621 gccttgatcg ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca
1681 cgatgcctgt agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc
1741 tagcttcccg gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc
1801 tgcgctcggc ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg
1861 ggtctcgcgg tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta
1921 tctacacgac ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag
1981 gtgcctcact gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga
2041 ttgatttaaa acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc
2101 tcatgaccaa aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa
2161 agatcaaagg atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa
2221 aaaaaccacc gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc
2281 cgaaggtaac tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt
2341 agttaggcca ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc
2401 tgttaccagt ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac
2461 gatagttacc ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca
2521 gcttggagcg aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg
2581 ccacgcttcc cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag
2641 gagagcgcac gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt
2701 ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat
2761 ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc
2821 acatgttctt tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt
2881 gagctgatac cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag
2941 cggaagagcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca
3001 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag tatacactcc
3061 gctatcgcta cgtgactggg tcatggctgc gccccgacac ccgccaacac ccgctgacgc
3121 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
3181 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgaggc agcagatcaa
3241 ttcgcgcgcg aaggcgaagc ggcatgcatt tacgttgaca ccatcgaatg gcgcaaaacc
3301 tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
3361 ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
3421 gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
3481 gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
3541 ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
3601 attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
3661 ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
3721 atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
3781 gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
3841 catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
3901 gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
3961 aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
4021 atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
4081 ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
4141 cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
4201 atcccgccgt caaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
4261 cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
4321 ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
4381 gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
4441 caacgcaatt aatgtgagtt agcgcgaatt gatctt
风险提示：丁香通仅作为第三方平台，为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全，合理判断，谨慎选购商品，商家和用户对交易行为负责。对于医疗器械类产品，请先查证核实企业经营资质和医疗器械产品注册证情况。
文献和实验相关实验
A Cell Architecture Modeling System Based on Quantitative Ultrastructural Characteristics
and therefore should include specific authoring tools such as appropriate visualization and stereological measures. For this purpose we have developed a problem solving environment for stereology-based modeling (PSE-SBM), which is an automated system for quantitative modeling
测定鱼中二噁英PCDDs/PCDFs、类二噁英多氯联苯、非类二噁英多氯联苯
比较快速溶剂萃取和传统索氏萃取 摘要对比快速溶剂萃取PSE (SpeedExtractor E-914)和传统索氏萃取方法，研究表明快速溶剂萃取PSE (SpeedExtractor E-914)能够高效地萃取测定鱼中的17个二噁英PCDD/Fs， 12个类二噁英和6个非类二噁英多氯联苯。脂肪含量不同的鱼样品（鳗鱼和鲑鳟鱼）中分离PCDD/Fs和PCBs。经过多步色谱纯化后，萃取物通过HRGC/HRMS分析。快速溶剂萃取PSE和索氏萃取对于标样鲑鳟鱼萃取结果都有准确
Se-Derivatized RNAs for X-ray Crystallography
analogs (Se-NTPs) and their use in vitro transcription and purification of Se-derivatized RNA samples (phosphoroselenoate RNA, PSe-RNA).
技术资料暂无技术资料 索取技术资料
pSE380
¥800 - 1200
