pGFPuv

pGFPuv

载体基本信息

出品公司:	Ybscience
载体名称:	pGFPuv
质粒类型:	大肠杆菌表达载体；基因载体
高拷贝/低拷贝:	高
启动子:	lac
克隆方法:	多克隆位点，限制性内切酶
载体大小:	3337 bp
5' 测序引物及序列:	lac_promoter: CACTTTATGCTTCCGGCTCG
3' 测序引物及序列:	--
载体标签:
载体抗性:	Ampicillin
筛选标记:	--
备注:	含有GFPuv基因
稳定性:	--
组成型:	--
病毒/非病毒:	--

 

载体质粒图谱和多克隆位点信息

载体简介

pGFPuv carries the“cycle 3”variant of GFP described by Crameri et al. (1). This gene was cloned between the two MCSs of the pUC19 derivative pPD16.43 (2). The GFPuv gene canbe easily excised from pGFPuv. Alternatively, the GFPuv coding sequence can be amplified by PCR. The GFPuv gene was inserted in frame with the lacZ initiation codon from pUC19 so that a β-galactosidase-GFPuv fusionprotein is expressed from the lac promoter in E. coli . Note, however, that if you excise the GFPuv coding sequence using a restriction site in the 5' MCS, the resulting fragment will encode the native (i.e.,non-fusion) GFPuv protein. The pUC backbone of pGFPuv provides a high copy number origin of replication and ampicillin resistance gene for propagation in E. coli.

载体序列

LOCUS       pGFPuv	3337 bp 	DNA	SYN
DEFINITION  pGFPuv
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..3337
                     /organism="pGFPuv"
                     /mol_type="other DNA"
     promoter        143..172
                     /label="lac_promoter"
     misc_feature    186..208
                     /label="M13_pUC_rev_primer"
     promoter        207..225
                     /label="M13_reverse_primer"
     CDS             217..1005
                     /label="ORF frame 1"
     gene            295..990
                     /label="GFP (0 - 695)"
                     /gene="GFP (0 - 695)"
     misc_feature    complement(319..347)
                     /label="GFP_R_primer"
     misc_feature    complement(363..382)
                     /label="GFPfusionREV_primer"
     misc_feature    942..962
                     /label="GFP_F_primer"
     misc_feature    complement(1127..1149)
                     /label="pGEX_3_primer"
     promoter        1467..1495
                     /label="AmpR_promoter"
     gene            1537..2397
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             1537..2397
                     /label="ORF frame 1"
     rep_origin      2552..3171
                     /label="pBR322_origin"
ORIGIN
    1 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
   61 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
  121 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
  181 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
  241 catgcctgca ggtcgactct agaggatccc cgggtaccgg tagaaaaaat gagtaaagga
  301 gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
  361 cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
  421 aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
  481 tcttatggtg ttcaatgctt ttcccgttat ccggatcata tgaaacggca tgactttttc
  541 aagagtgcca tgcccgaagg ttatgtacag gaacgcacta tatctttcaa agatgacggg
  601 aactacaaga cgcgtgctga agtcaagttt gaaggtgata cccttgttaa tcgtatcgag
  661 ttaaaaggta ttgattttaa agaagatgga aacattctcg gacacaaact cgagtacaac
  721 tataactcac acaatgtata catcacggca gacaaacaaa agaatggaat caaagctaac
  781 ttcaaaattc gccacaacat tgaagatgga tccgttcaac tagcagacca ttatcaacaa
  841 aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtcgacacaa
  901 tctgcccttt cgaaagatcc caacgaaaag cgtgaccaca tggtccttct tgagtttgta
  961 actgctgctg ggattacaca tggcatggat gagctctaca aataatgaat tccaactgag
 1021 cgccggtcgc taccattacc aacttgtctg gtgtcaaaaa taataggcct actagtcggc
 1081 cgtacgggcc ctttcgtctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc
 1141 agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc
 1201 agggcgcgtc agcgggtgtt ggcgggtgtc ggggctggct taactatgcg gcatcagagc
 1261 agattgtact gagagtgcac catatgcggt gtgaaatacc gcacagatgc gtaaggagaa
 1321 aataccgcat caggcggcct taagggcctc gtgatacgcc tatttttata ggttaatgtc
 1381 atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc
 1441 cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc
 1501 tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc
 1561 gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg
 1621 gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat
 1681 ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc
 1741 acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa
 1801 ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa
 1861 aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt
 1921 gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct
 1981 tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat
 2041 gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg
 2101 cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg
 2161 atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt
 2221 attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg
 2281 ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg
 2341 gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg
 2401 tcagaccaag tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa
 2461 aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt
 2521 tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt
 2581 tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt
 2641 ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag
 2701 ataccaaata ctgtccttct agtgtagccg tagttaggcc accacttcaa gaactctgta
 2761 gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat
 2821 aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg
 2881 ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg
 2941 agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac
 3001 aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga
 3061 aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt
 3121 ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta
 3181 cggttcctgg ccttttgctg gccttttgct cacatgttct ttcctgcgtt atcccctgat
 3241 tctgtggata accgtattac cgcctttgag tgagctgata ccgctcgccg cagccgaacg
 3301 accgagcgca gcgagtcagt gagcgaggaa gcggaag