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pET-14b

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  • ¥800 - 1200
  • Ybscience
  • 中国/美国
  • YB-0108
  • 2025年07月12日
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    • 文献和实验
    • 技术资料
    • 保存条件

      -20℃

    • 保质期

      6个月

    • 英文名

      pET-14b

    • 库存

      大量

    • 供应商

      钰博生物

    • 规格

      1ug/5ug

    pET-14b

    pET14b载体基本信息

    出品公司: Ybscience
    别名: pET14b, pet 14b
    质粒类型: 大肠杆菌蛋白表达
    表达水平:
    克隆方法: 多克隆位点,限制性内切酶
    载体大小: 4671bp
    5' 测序引物: T7
    5' 测序引物序列:   5'-TAATACGACTCACTATAGGG-3'
    载体标签: N-His, N-thrombin
    载体抗性: Ampicillin
    备注: Hosts: E.coli. Related vectors: pBR322.
    稳定性: 瞬时表达 Transient
    组成型: 组成型 Constitutive
    病毒/非病毒: 非病毒

    pET14b载体质粒图谱和多克隆位点信息

    pET-14b载体图谱



    pET-14b多克隆位点

    pET-14b载体特征

    Feature Name Start End  
    tet (w/ gaps) 86 1584
    T7_terminator 507 379
    T7_Terminal_primer 447 465
    T7_transl_en_RBS 607 591
    T7_promoter 662 644
    pBRrevBam_primer 765 746
    ROP 2223 2414
    pGEX_3_primer 2430 2408
    Ampicillin 4463 3603
    AmpR_promoter 4533 4505
    ORF Start End  
    ORF frame 1 799 1584
    ORF frame 1 1389 931
    ORF frame 2 4463 3603
    Enzyme Name Cut
    ClaI 24
    HindIII 29
    NheI 229
    BamHI 510
    XhoI 515
    NdeI 522
    NcoI 580
    XbaI 619
    BglII 677
    SalI 959
    EagI 1247
    NruI 1282
    MscI 1754
    PvuII 2374
    PstI 3921
    AatII 4598
    EcoRI 4669

     

    pET14b载体简介

     

    The pET-14b vector (Cat. No. 69660-3) carries an N-terminal His•Tag® sequence followed by a thrombin site and three cloning sites. Unique sites are shown on the circle map. Note that thesequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below.
    
    
    pET Vectors 选择指南
     

     

    pET14b载体序列

    LOCUS       pET-14b    4671 bp     DNA  circular    SYN
    DEFINITION  pET-14b
    ACCESSION   
    KEYWORDS    
    SOURCE      
      ORGANISM  other sequences; artificial sequences; vectors.
    COMMENT     This file is created by Vector NTI
                http://www.biofeng.com/
    COMMENT     ORIGDB|GenBank
    COMMENT     VNTAUTHORNAME|biofeng.com|
    COMMENT     VNTOAUTHORAD1|Novagen Inc.|
    FEATURES             Location/Qualifiers
         source          1..4671
                         /organism="pET-14b"
                         /mol_type="other DNA"
         gene            86..1584
                         /label="tet (w/ gaps)"
                         /gene="tet (w/ gaps)"
         terminator      complement(379..507)
                         /label="T7_terminator"
         misc_feature    447..465
                         /label="T7_Terminal_primer"
         misc_feature    complement(591..607)
                         /label="T7_transl_en_RBS"
         promoter        complement(644..662)
                         /label="T7_promoter"
         misc_feature    complement(746..765)
                         /label="pBRrevBam_primer"
         CDS             799..1584
                         /label="ORF frame 1"
                         /translation="MSACFGVGMVAGPVAGGLLGAISLHAPFLAAAVLNGLNLLLGCF
                         LMQESHKGERRPMPLRAFNPVSSFRWARGMTIVAALMTVFFIMQLVGQVPAALWVIFG
                         EDRFRWSATMIGLSLAVFGILHALAQAFVTGPATKRFGEKQAIIAGMAADALGYVLLA
                         FATRGWMAFPIMILLASGGIGMPALQAMLSRQVDDDHQGQLQGSLAALTSLTSITGPL
                         IVTAIYAASASTWNGLAWIVGAALYLVCLPALRRGAWSRATST*"
         CDS             complement(931..1389)
                         /label="ORF frame 1"
                         /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                         RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                         CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
         misc_feature    2223..2414
                         /label="ROP"
                         /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                         RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                         CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
         misc_feature    complement(2408..2430)
                         /label="pGEX_3_primer"
                         /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                         RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                         CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
         gene            complement(3603..4463)
                         /label="Ampicillin"
                         /gene="Ampicillin"
                         /translation="MVVIYLPGQHGLQRGHPDAAGSEKNHNGEGHPASRRERQQDVAQ
                         RVGRHAGDNGLLLAETFGGGTSDEGLSEGVQDSEYRKRQADHRRAPAKAVLAENDPER
                         CRHLSYELHDKEDSHKCGDDSHAPRPPEGADWVEGSQGHRSTLSLMRLLH*"
         CDS             complement(3603..4463)
                         /label="ORF frame 2"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
         promoter        complement(4505..4533)
                         /label="AmpR_promoter"
                         /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGY
                         IELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVE
                         YSPVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRL
                         DRWEPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPL
                         LRSALPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIA
                         EIGASLIKHW*"
    ORIGIN
        1 ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
       61 ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
      121 caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
      181 gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
      241 tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
      301 ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
      361 cacacccgtc ctgtggatat ccggatatag ttcctccttt cagcaaaaaa cccctcaaga
      421 cccgtttaga ggccccaagg ggttatgcta gttattgctc agcggtggca gcagccaact
      481 cagcttcctt tcgggctttg ttagcagccg gatcctcgag catatggctg ccgcgcggca
      541 ccaggccgct gctgtgatga tgatgatgat ggctgctgcc catggtatat ctccttctta
      601 aagttaaaca aaattatttc tagagggaaa ccgttgtggt ctccctatag tgagtcgtat
      661 taatttcgcg ggatcgagat ctcgatcctc tacgccggac gcatcgtggc cggcatcacc
      721 ggcgccacag gtgcggttgc tggcgcctat atcgccgaca tcaccgatgg ggaagatcgg
      781 gctcgccact tcgggctcat gagcgcttgt ttcggcgtgg gtatggtggc aggccccgtg
      841 gccgggggac tgttgggcgc catctccttg catgcaccat tccttgcggc ggcggtgctc
      901 aacggcctca acctactact gggctgcttc ctaatgcagg agtcgcataa gggagagcgt
      961 cgaccgatgc ccttgagagc cttcaaccca gtcagctcct tccggtgggc gcggggcatg
     1021 actatcgtcg ccgcacttat gactgtcttc tttatcatgc aactcgtagg acaggtgccg
     1081 gcagcgctct gggtcatttt cggcgaggac cgctttcgct ggagcgcgac gatgatcggc
     1141 ctgtcgcttg cggtattcgg aatcttgcac gccctcgctc aagccttcgt cactggtccc
     1201 gccaccaaac gtttcggcga gaagcaggcc attatcgccg gcatggcggc cgacgcgctg
     1261 ggctacgtct tgctggcgtt cgcgacgcga ggctggatgg ccttccccat tatgattctt
     1321 ctcgcttccg gcggcatcgg gatgcccgcg ttgcaggcca tgctgtccag gcaggtagat
     1381 gacgaccatc agggacagct tcaaggatcg ctcgcggctc ttaccagcct aacttcgatc
     1441 actggaccgc tgatcgtcac ggcgatttat gccgcctcgg cgagcacatg gaacgggttg
     1501 gcatggattg taggcgccgc cctatacctt gtctgcctcc ccgcgttgcg tcgcggtgca
     1561 tggagccggg ccacctcgac ctgaatggaa gccggcggca cctcgctaac ggattcacca
     1621 ctccaagaat tggagccaat caattcttgc ggagaactgt gaatgcgcaa accaaccctt
     1681 ggcagaacat atccatcgcg tccgccatct ccagcagccg cacgcggcgc atctcgggca
     1741 gcgttgggtc ctggccacgg gtgcgcatga tcgtgctcct gtcgttgagg acccggctag
     1801 gctggcgggg ttgccttact ggttagcaga atgaatcacc gatacgcgag cgaacgtgaa
     1861 gcgactgctg ctgcaaaacg tctgcgacct gagcaacaac atgaatggtc ttcggtttcc
     1921 gtgtttcgta aagtctggaa acgcggaagt cagcgccctg caccattatg ttccggatct
     1981 gcatcgcagg atgctgctgg ctaccctgtg gaacacctac atctgtatta acgaagcgct
     2041 ggcattgacc ctgagtgatt tttctctggt cccgccgcat ccataccgcc agttgtttac
     2101 cctcacaacg ttccagtaac cgggcatgtt catcatcagt aacccgtatc gtgagcatcc
     2161 tctctcgttt catcggtatc attaccccca tgaacagaaa tcccccttac acggaggcat
     2221 cagtgaccaa acaggaaaaa accgccctta acatggcccg ctttatcaga agccagacat
     2281 taacgcttct ggagaaactc aacgagctgg acgcggatga acaggcagac atctgtgaat
     2341 cgcttcacga ccacgctgat gagctttacc gcagctgcct cgcgcgtttc ggtgatgacg
     2401 gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     2461 ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag
     2521 ccatgaccca gtcacgtagc gatagcggag tgtatactgg cttaactatg cggcatcaga
     2581 gcagattgta ctgagagtgc accatatatg cggtgtgaaa taccgcacag atgcgtaagg
     2641 agaaaatacc gcatcaggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     2701 gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     2761 tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     2821 aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     2881 aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     2941 ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     3001 tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc
     3061 agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     3121 gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     3181 tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     3241 acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     3301 tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     3361 caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     3421 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     3481 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     3541 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     3601 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     3661 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     3721 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     3781 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     3841 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     3901 aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     3961 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     4021 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     4081 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     4141 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     4201 tgctcttgcc cggcgtcaac acgggataat accgcgccac atagcagaac tttaaaagtg
     4261 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     4321 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     4381 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     4441 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     4501 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     4561 gttccgcgca catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg
     4621 acattaacct ataaaaatag gcgtatcacg aggccctttc gtcttcaaga a

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