手机验证
-20℃
6个月
pET-23(+)
大量
钰博生物
1ug/5ug
出品公司: | Ybscience |
---|---|
载体名称: | pET-23a |
质粒类型: | 大肠杆菌表达载体;蛋白纯化 |
高拷贝/低拷贝: | 低拷贝 |
克隆方法: | 多克隆位点;限制性内切酶 |
启动子: | T7/ lacI |
载体大小: | 3592 bp |
5' 测序引物及序列: | T7 Fwd: 5'd[TAATACGACTCACTATAGGG]3' |
3' 测序引物及序列: | -- |
载体标签: | His Tag (C-端) |
载体抗性: | 氨苄青霉素(Ampicillin) |
克隆菌株: | DH5a |
表达菌株: | BL21系列 |
备注: | pET-23a,b,c仅阅读框(读码框)不同,彼此相差1个bp。 |
稳定性: | 稳表达 |
组成型/诱导型: | 诱导型(IPTG) |
病毒/非病毒: | 非病毒 |
Feature Name | Start | End | |
---|---|---|---|
T7_terminator | 129 | 1 | |
T7_Terminal_primer | 69 | 87 | |
T7_promoter | 245 | 227 | |
ROP | 754 | 945 | |
pGEX_3_primer | 961 | 939 | |
pBR322_origin | 1979 | 1360 | |
Ampicillin | 2994 | 2134 | |
AmpR_promoter | 3064 | 3036 |
ORF | Start | End | |
---|---|---|---|
ORF frame 2 | 2994 | 2134 |
Enzyme Name | Cut |
---|---|
XhoI | 158 |
NotI | 166 |
EagI | 166 |
HindIII | 173 |
SalI | 179 |
SacI | 190 |
EcoRI | 192 |
BamHI | 198 |
BglII | 260 |
MscI | 285 |
PvuII | 905 |
PstI | 2452 |
pET-23(+) (Cat. No. 69771-3) is a transcription vector designed for expression from bacterial translation signals carried within a cloned insert. It therefore lacks the ribosome binding site and ATG start codon present on the pET translation vectors. A C-terminal His•Tag® sequence is available. Unique sites are shown on the circle map. Note that the sequence is numbered by the pBR322 convention, so the T7 expression region is reversed on the circular map. The cloning/expression region of the coding strand transcribed by T7 RNA polymerase is shown below. The f1 origin is oriented so that infection with helper phage will produce virions containing single-stranded DNA that corresponds to the coding strand. Therefore, single-stranded sequencing should be performed using the T7 terminator primer (Cat. No. 69337-3).
风险提示:丁香通仅作为第三方平台,为商家信息发布提供平台空间。用户咨询产品时请注意保护个人信息及财产安全,合理判断,谨慎选购商品,商家和用户对交易行为负责。对于医疗器械类产品,请先查证核实企业经营资质和医疗器械产品注册证情况。