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- A5602-5MG
- 2025年11月07日
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- 保存条件：
常温
- 库存：
现货
- 供应商：
联硕
- 规格：
5mg
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生化/生理作用
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文献和实验Dissociated Retina Immunohistochemistry
Solutions 100X trypsin 10 mg/ml in 1X PBS Sigma #T2271 100X soybean trypsin inhibitor 10 mg/ml in 1X PBS Sigma #T6522 1000X DNaseI 2mg/ml in HBSS Sigma #D4513 Explant Culture
Establishment of Stable Transfectant of CHO Lec Cells
expression vectors with strong promoter such as immediate early CMV (pcDNA3.1 etc.) or human EF-1a (pEF1/V5-His etc.). pBJ-5/GS vector also gives you high expression but requires additional cloning strategy. Materials Medium Sigma provides
Establishment of Stable Transfectant of CHO Lec Cells
) by yourself but this cannot be used as serum-free medium. Recipe for 10 lt of J/J medium: To 9 lt of mili-Q water, add MEM w/o Gln (GIBCO, #11700-077). Pwdr for 10 lt. NaHCO3 , 27.75g glucose: 30 g nucleosides: adenosine (Sigma A-4036), 70mg
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