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- 详细信息
- 文献和实验
- 技术资料
- 免疫原:
Native human SP-D purified from late pregnancy amniotic fluid
- 亚型:
Mouse IgG2a/k
- 形态:
Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography.
- 保存条件:
Store at 4°C.
- 克隆性:
单克隆抗体
- 标记物:
见下方详细说明
- 适应物种:
H
- 宿主:
Mouse
- 应用范围:
E, WB
- 浓度:
见详细说明信息
- 靶点:
Surfactant protein D (10B2)
- 抗体英文名:
Surfactant protein D (10B2) Antibody
- 抗体名:
Surfactant protein D (10B2)抗体
- 规格:
0.2 mg
Surfactant protein D (10B2) Antibody产品详细介绍:
| 产品名称: | Surfactant protein D (10B2) Antibody |
| 说明书: | http://www.abbiotec.com/antibodies/surfactant-protein-d-10b2-antibody【参考图片】 |
| 货号: | 250403 |
| 规格: | 0.2 mg |
| 产品描述: | Surfactant protein D (SP-D) is synthesized and secreted by lung epithelial cells. It belongs to group III of the family of C-type lectins and members of this group has overall structure consisting of multiple globular 'head' regions linked by triple-helical, collagen-like, strands. This group also includes SP-A and the serum proteins mannan-binding protein, conglutinin and collectin-43, all of which have been shown to bind to the C1q receptor found on a wide variety of cells. Both SP-D and SP-A have been shown to enhance oxygen radical production by alveolar macrophages. The serum concentration is approximately 700 ng/ml in healthy individuals. This antibody is specific for SP-D from amniotic fluid, serum and bronchioalveolar lavage. The antibody blocks SP-D binding to GP-340 but not to carbohydrates. |
| 克隆类型: | Mouse Monoclonal Antibody |
| 亚型: | Mouse IgG2a/k |
| 免疫原: | Native human SP-D purified from late pregnancy amniotic fluid |
| 适用物种: | H |
| 应用范围: | E, WB |
| 应用说明: | Product Cat. No. 250403 is used as detection antibody in sandwich ELISAwith a polyclonal antibody. Antibody binding is calcium-dependent. Product Cat. No. 250403 only reacts in western blot with non-reduced SP-D. E: 1:2,000; WB: 1:2,000 |
| 别名: | Pulmonary surfactant-associated protein D; SP-D; PSP-D; PSPD; Lung surfactant protein D |
| 形态: | Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography. |
| 保存条件: | Store at 4°C. Product is guaranteed 6 months from the date of shipment. |
| 参考文献: |
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文献和实验Purification of Surfactant Protein D (SP-D) from Pooled Amniotic Fluid and Bronchoalveolar Lavage
Surfactant protein SP-D is a multimeric collagenous lectin, called collectin . SP-D is a multifunctional, pattern recognition innate immune molecule, which binds in a calcium dependent manner to an array of carbohydrates and lipids
Analysis of Protein Complexes by 2D Blue Native/SDSPAGE and Antibody-Shift Assay
Blue native polyacrylamide gel electrophoresis (BN-PAGE) is a powerful strategy for the separation of native multiprotein complex (MPC). Combined with other techniques, such as SDS–PAGE, immunoblotting, mass spectrometry, and antibody-shift
ANTIBODY BINDING TO PROTEIN A AND PROTEIN G
. A physicochemical study f protein G, a molecule with unique immunoglobulin G-binding properties. J. Biol. Chem. 261:10240-10247. Harlow, E. and Lane, D. 1988. Antibodies. A laboratory manual. Cold Spring Harbor Laboratory. Kronvall, G., Seal, U. S
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