- ￥3000 - 3999
- Abbiotec
- 美国
- 250385
- 2025年07月12日
- E, WB, IHC
- Mouse
- H
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
Human recombinant NGAL in monomer and dimer forms adsorbed to aluminium hydroxide gel.
- 亚型：
Mouse IgG1
- 形态：
Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography.
- 保存条件：
Store at 4°C.
- 克隆性：
单克隆抗体
- 标记物：
见下方详细说明
- 适应物种：
H
- 宿主：
Mouse
- 应用范围：
E, WB, IHC
- 浓度：
见详细说明信息
- 靶点：
NGAL (5G5)
- 抗体英文名：
NGAL (5G5) Antibody
- 抗体名：
NGAL (5G5)抗体
- 规格：
0.2 mg
NGAL (5G5) Antibody产品详细介绍：
| 产品名称： | NGAL (5G5) Antibody |
| 说明书： | http://www.abbiotec.com/antibodies/ngal-5g5-antibody【参考图片】 |
| 货号： | 250385 |
| 规格： | 0.2 mg |
| 产品描述： | Neutrophil gelatinase-associated lipocalin (NGAL; also called lipocalin 2, siderocalin and neutrophil lipocalin) is a member of the lipocalin family of proteins which bind and transport small lipophilic molecules. NGAL is released by activated neutrophils and occurs as 25-kDa glycosylated single protein chain monomers, which form dimers and small amounts of higher oligomers, as well as complexes with matrix metalloproteinase 9 (MMP-9; gelatinase B). Low level expression of NGAL in a variety of epithelia may be increased in inflammation or cancers. The expression and release of NGAL from renal tubules are dramatically increased by ischemic or nephrotoxic injury. This antibody is specific for human NGAL. No cross-reaction with NGAL from other species has been detected. |
| 克隆类型： | Mouse Monoclonal Antibody |
| 亚型： | Mouse IgG1 |
| 免疫原： | Human recombinant NGAL in monomer and dimer forms adsorbed to aluminium hydroxide gel. |
| 适用物种： | H |
| 应用范围： | E, WB, IHC |
| 应用说明： | Product Cat. No. 250385 reacts strongly in ELISA with human NGAL coated directly onto the well, or in sandwich ELISA used as detection antibody with a polyclonal capture antibody. Product Cat. No. 250385 reacts with NGAL in immunochemical staining of paraformaldehyde-fixed sections containing neutrophils. Product Cat. No. 250385 reacts in western blot with NGAL in both reduced and non-reduced forms. E: 1:16,000; WB: 1:500; IHC: 1:500 |
| 别名： | Neutrophil gelatinase-associated lipocalin; ; 25; 25 kDa alpha-2-microglobulin-related subunit of MMP-9; Lipocalin-2; Oncogene 24p3 |
| 形态： | Each vial contains 0.2 mg IgG in 0.2 ml (1 mg/ml) of PBS pH7.4 with 0.09% sodium azide. Antibody was purified by Protein-A/G affinity chromatography. |
| 保存条件： | Store at 4°C. Product is guaranteed 6 months from the date of shipment. |
| 参考文献： |
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大鼠Lipocalin-2 (NGAL)ELISA试剂盒 说明书
上海西唐生物科技有限公司 021-55229872, 65333639 www.westang.com 大鼠 Lipocalin-2 (NGAL)ELISA 试剂盒 ( 用于血清、血浆、细胞培养上清液和尿液生物体液内 ) 原理 本实验采用双抗体夹心 ABC-ELISA 法。用抗大鼠 NGAL 单抗包被于酶标板上，标准品和样品中的 NGAL 与单抗结合，加入生物素化的抗大鼠 NGAL ，形成免疫复合
这个标记内参的10次使用装) 图示: A-G分别表示不同实验小鼠心脏蛋白(上样量50ug)，兔抗小鼠Akt抗体(康成生物Cat#KC-5A01)检测心脏组织匀浆中Akt水平。加入兔二抗(康成生物Cat#KC-RB-035，稀释比例为1：5，000)时同时加入HRP标记的抗GAPDH单抗(稀释比例为1：10，000，康成生物Cat#KC-5G5)。采用化学发光试剂盒(康成生物Cat#KC-420)及X胶片曝光显影。一次反应即可同时检测目的蛋白(Akt)与内参GAPDH的含量。
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