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- 美国莱伯特（Labnet）
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- E1010-10-GT
- 2025年07月12日
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文献和实验and solution is clear. Allow solution to cool to about 55EC before pouring. (Ethidium bromide can be added at this point to a concentration of 0.5 μg/ml) Prepare gel tray by sealing ends with tape or other custom-made dam. Place comb
SDS Gel Electrophoresis of Tubulin\MAPs
% TCA Procedure Prepare 15 cm glass tubes with 6 mm internal diameter. Polymerize a 10 cm separation gel and a 1 cm stacking gel within the tubes by the addition of TEMED and ammonium persulfate as directed in Chapter Four.
A protocol for cleaning and reusing the large 25 x 25 cm plates
We regularly reuse our large 25x25 cm plating trays; initially, however, we were plagued by gross microbiological contamination when reusing the trays. Various combinations of cleaning with a laboratory dishwasher, ethyl alcohol, and UV
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