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- 美国莱伯特（Labnet）
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- D0461
- 2025年07月10日
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文献和实验A general protocol for staining cell for cytometry analysis
mix the cells and incubate for 15 min at RT in the dark. *For Annexin V-Biotin samples only: After 15 min incubation, wash once with 1 ml of 1X Binding Buffer. Dilute 0.5 µg Streptavidin-Fluorescein Isothiocynate (SAv-FITC, Cat. No. 13024D) into 100
A general protocol for staining cell for cytometry analysis
-Biotin samples only: After 15 min incubation, wash once with 1 ml of 1× Binding Buffer. Dilute 0.5 µg Streptavidin-Fluorescein Isothiocynate (SAv-FITC, Cat. No. 13024D) into 100 µl of 1× Binding Buffer and add to the cell pellet. Gently mix the cells. Add
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
histochemical reporter gene (Beckwith, 1980). It can be detected using a variety of substrates, all of which have galactose linked through a ß-D-glycosidic linkage to a moiety whose properties change upon liberation from galactose (Wallenfels and Weil, 1972
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