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文献和实验secondary antibody review -- data from 99 publications
cytometry used as a control to detect cell responses targeted antigen 7 Alexa Fluor 488 7 Cy3 8 goat IgG Alexa Fluor 488 1:2000 detect antibody binding in human embryonic kidney 293T cells Invitrogen 9 donkey
IMMUNOSCREENING OF WITH ANTIBODY PROBES
in 10mM IPTG (MW=238.31). Briefly blot the excess solution from the filters, and wrap them in cellophane. These should be moist and stored at 4°C. We typically adsorb serum with five 150 mm Petri dishes of lysed E. coli to reduce anti-E. coli antibody
Antibody Staining of Imaginal Discs
minutes each wash. 7. Replace last wash with 452 ul Tri-PBS, 5 ul 10% BSA, and 40 ul normal goat serum (NGS); rock for 30 minutes. 8. Add primary antibody directly to the tube and rock overnight at room temperature. 9. Wash tissues 5 times
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