Recombinant Human LAG-3/CD223

Recombinant Human LAG-3/CD223  (CU29)

产品说明(Description)

Recombinant Human Lymphocyte Activation Gene 3 Protein is produced by our Mammalian expression system and the target gene encoding Leu23-Leu450 is expressed with a 6His tag at the C-terminus. 

Accession #: P18627

Known as: Lymphocyte activation gene 3 protein; LAG3; LAG-3; Protein FDC; CD223

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of PBS, 2mM EDTA, pH7.4.

 

质量控制(Quality Control) 

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/µg (1 EU/µg) as determined by LAL test.

 

Bioactivity-BLI:
Loaded Anti-Human LAG-3 mAb-Fc(Cat#NC067) on Protein A Biosensor, can bind Human LAG-3-6His(Cat#CU29) with an affinity constant of 0.41 nM as determined in BLI assay.

 

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Human Lymphocyte activation gene 3 protein( LAG3) is a member of immunoglobulin (Ig) superfamily. LAG3 contains 4 extracellular Ig-like domains. The LAG3 gene contains 8 exons. LAG3 is involved in lymphocyte activation and can bind to HLA class-II antigens. It is selectively expressed in activated T and NK cells. LAG3 has a negative regulatory function in T cells and acts as as a new marker of T cell induced B cell activation. As a soluble molecule, LAG3 activates antigen-presenting cells through MHC class II signaling. It can lead to increased antigen-specific T-cell responses in vivo. LAG-3 has higher affinity to MHC class II than CD4.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY