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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Recombinant Human ACPP/Prostatic Acid Phosphatase
- 规格:
10ug/50ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥1200.0 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥3520.0 |
| 规格: | 500ug | 产品价格: | 询价 |
| 规格: | 1mg | 产品价格: | 询价 |
Recombinant Human ACPP/Prostatic Acid Phosphatase (C420)
产品说明(Description)
Recombinant Human Prostatic Acid Phosphatase is produced by our Mammalian expression system and the target gene encoding Lys33-Asp386 is expressed with a 6His tag at the C-terminus.
Accession #: AAH16344.1
Known as: Prostatic Acid Phosphatase; PAP; 5'-Nucleotidase; 5'-NT, Ecto-5'-Nucleotidase; Thiamine Monophosphatase; TMPase; ACPP
制剂(Formulation)
Supplied as a 0.2 μm filtered solution of 20mM TrisHCl, 150mM NaCl, pH 7.5.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
运输(Shipping)
The product is shipped on dry ice/polar packs.
Upon receipt, store it immediately at the temperature listed below.
保存(Storage)
Store at ≤-70°C, stable for 6 months after receipt.
Store at ≤-70°C, stable for 3 months under sterile conditions after opening.
Please minimize freeze-thaw cycles.
背景(Background)
Prostatic Acid Phosphatase (PAP) belongs to the histidine acid phosphatase family. PAP can catalyze the hydrolysis of member of phosphate monoestyers, including phosphorylated protein. PAP can high expression in metastasized prostate cancer, moderately expression level in bone diseases, blood cell disease, and the concentration of PAP is used to monitor and assess the proession of prostate cancer. The optimum PH of PAP is from 4 to 6; its activity can be inhibited by L(+)-tartrate.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY
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文献和实验Important aspects of the relatively new technique, nucleic acid immunocytochemistry, are predicated on principles that are different from those that underlie the immunocytochemistry protocols described in earlier chapters of this book
In Situ Hybridization to Human Chromosomes of an Alkaline Phosphatase-Labeled Centromeric Probe
The application of in situ hybridization and the underlying rationale are described in Chapter 46. In situ hybridization of nucleic acid probes to cellular targets of biological specimens affixed to a slide has benefited greatly
Assaying Cdc25 Phosphatase Activity
To determine the activity of all human Cdc25 phosphatases, two different methods are described. For assaying phosphatase activities of recombinant Cdc25 proteins produced in Escherichia coli or insect cells, a fluorimetric assay using
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