Recombinant Mouse Cell adhesio

Recombinant Mouse Cell adhesion molecule 1/CADM1  (C759)

 

产品说明(Description)

Recombinant Mouse Cell adhesion molecule 1 is produced by our Mammalian expression system and the target gene encoding Gln48-His388 is expressed with a Fc tag at the C-terminus. 

Accession #: Q8R5M8

Known as: Cell adhesion molecule 1;Immunoglobulin superfamily member 4;IgSF4;Nectin-like protein 2;NECL-2;Synaptic cell adhesion molecule;SynCAM;Tumor suppressor in lung cancer 1;TSLC-1

 

制剂（Formulation）

Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

 

质量控制（Quality Control） 

 

Purity:            Greater than 90% as determined by reducing SDS-PAGE.    

Endotoxin:    Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

Immobilized Human CADM1-Fc (Cat#C759) at 2μg/ml (100 μl/well) can bind Human CRTAMV-His(Cat#C331).The ED50 of Human CRTAMV-His(Cat#C331) is 4.905 ug/ml .

 

复溶（Reconstitution）

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存（Storage）

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景（Background）

Cell adhesion molecule 1(CADM1)  is a single-pass type I membrane protein and belongs to the nectin family. It contains 2 Ig-like C2-type (immunoglobulin-like) domains and 1 Ig-like V-type (immunoglobulin-like) domain. CADM1 acts as a tumor suppressor in non-small-cell lung cancer (NSCLC) cells. Interaction with CRTAM promotes natural killer (NK) cell cytotoxicity and interferon-gamma (IFN-gamma) secretion by CD8+ cells in vitro as well as NK cell-mediated rejection of tumors expressing CADM3 in vivo. CADM1 may contribute to the less invasive phenotypes of lepidic growth tumor cells. In mast cells, it may mediate attachment to and promote communication with nerves. CADM1, together with MITF, is essential for development and survival of mast cells in vivo. The protein acts as a synaptic cell adhesion molecule and plays a role in the formation of dendritic spines and in synapse assembly. It may be involved in neuronal migration, axon growth, pathfinding, and fasciculation on the axons of differentiating neurons. CADM1 may play diverse roles in the spermatogenesis including in the adhesion of spermatocytes and spermatids to Sertoli cells and for their normal differentiation into mature spermatozoa.

 

电泳(SDS-PAGE)

 

FOR RESEARCH USE ONLY