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Recombinant Mouse IL-7 (C-6His

)
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  • ¥1200 - 3520
  • 近岸蛋白(Novoprotein)已认证
  • CC73
  • 中国
  • 2025年11月16日
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    • 详细信息
    • 文献和实验
    • 技术资料
    • 英文名

      Recombinant Mouse IL-7 (C-6His)

    • 规格

      10ug/50ug/500ug/1mg

    规格:10ug产品价格:¥1200.0
    规格:50ug产品价格:¥3520.0
    规格:500ug产品价格:询价
    规格:1mg产品价格:询价

    Recombinant Mouse IL-7 (C-6His) (CC73)

    产品说明(Description)

    Recombinant Mouse Interleukin-7 is produced by our Mammalian expression system and the target gene encoding Glu26-Ile154 is expressed with a 6His tag at the C-terminus. 

    Accession #: P10168

    Known as: IL-7; IL-7 interleukin-7; interleukin-7; Lymphopoietin -1; PBGF

     

    制剂(Formulation)

    Lyophilized from a 0.2 μm filtered solution of PBS, pH7.4.

     

    质量控制(Quality Control) 

    Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

    Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

    Bioactivity: Measured in a cell proliferation assay using PHA-activated human peripheral blood lymphocytes (PBL). The ED50 for this effect is 60-1000 pg/ml.

     

    Bioactivity-BLI:    

    1615203328391473.jpg

       

    Loaded Mouse IL-7RA-Fc(Cat#CK77) on Protein A Biosensor, can bind Mouse IL-7-His(Cat#CC73) with an affinity constant of 18.9 nM as determined in BLI assay.

               

                                                                                                                                                                                              

     

    复溶(Reconstitution)

    Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
    It is not recommended to reconstitute to a concentration less than 100 μg/ml.

    Dissolve the lyophilized protein in distilled water.
    Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

     

    保存(Storage)

    Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.

    Reconstituted protein solution can be stored at 4-7°C for 2-7 days.

    Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

     

    背景(Background)

    Mouse interleukin-7(IL-7) is the member of hemopoietin family which is important to the differentiation, proliferation, and survival of lymphocyte. Mouse IL-7 shares approximately 88% aa sequence identity with rat IL-7 and 58-60% with human, equine, bovine, ovine, porcine, feline and canine IL-7. It is widely expressed in primary and secondary lymphoid tissues cell and stromal epithelial cells of the thymus, bone marrow, and intestines. IL-7 activation of IL-7 R alpha is critical for both T cell and B cell lineage development. It is important for proliferation during certain stages of B-cell maturation. IL-7 contributes to the maintenance of all naïve and memory T cells, mainly by promoting expression of the anti-apoptotic protein Bcl-2. It is required for optimal T cell-dendritic cell interaction.

     

    泳(SDS-PAGE)

     

    FOR RESEARCH USE ONLY

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