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- 详细信息
- 文献和实验
- 技术资料
- 英文名:
Recombinant Human ST2 (C-6His)
- 规格:
10ug/50ug/500ug/1mg
| 规格: | 10ug | 产品价格: | ¥400.0 |
|---|---|---|---|
| 规格: | 50ug | 产品价格: | ¥840.0 |
| 规格: | 500ug | 产品价格: | 询价 |
| 规格: | 1mg | 产品价格: | 询价 |
Recombinant Human ST2 (C-6His) (CI84)
产品说明(Description)
Recombinant Human Interleukin-1 receptor-like 1 is produced by our Mammalian expression system and the target gene encoding Lys19-Phe328 is expressed with a 6His tag at the C-terminus.
Accession #: Q01638-2
Known as: Interleukin-1 receptor-like 1;Protein ST2;DER4; ST2; T1
制剂(Formulation)
Lyophilized from a 0.2 μm filtered solution of 20mM PB,150mM NaCl,pH7.4.
质量控制(Quality Control)
Purity: Greater than 95% as determined by reducing SDS-PAGE.
Endotoxin: Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.
复溶(Reconstitution)
Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
保存(Storage)
Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.
背景(Background)
Interleukin 1 receptor-like 1(IL1RL1) is a member of the interleukin-1 receptor family, Contains 3 Ig-like C2-type domains and 1 TIR domain. It is highly expressed in kidney, lung, placenta, stomach, skeletal muscle, colon and small intestine. IL1RL1 is a receptor for interleukin-33, its stimulation recruits MYD88, IRAK1, IRAK4, and TRAF6, followed by phosphorylation of MAPK3/ERK1 and/or MAPK1/ERK2, MAPK14, and MAPK8. IL1RL1 may possibly be involved in helper T-cell function. Soluble IL1RL1 also acts as a negative regulator of Th2 cytokine production, it directly implicated in the progression of cardiac disease.
电泳(SDS-PAGE)
FOR RESEARCH USE ONLY
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文献和实验A general protocol for staining cell for cytometry analysis
according to cell type, and stage of apoptosis. In some cases, investigators may also need to reduce the number of cells to 0.5 x 10 5 /100 µl and still add 5-15 µg of recombinant Annexin V to obtain optimal results. 4. Gently mix the cells
DETECTION OF ß-GALACTOSIDASE AND ALKALINE PHOSPHATASE ACTIVITIES IN TISSUE
. Biol. In press, 1994. *Fields-Berry, S.C., A.L. Halliday, and C.L. Cepko. 1992. A recombinant retrovirus encoding alkaline phosphatase confirms clonal boundary assignment in lineage analysis of murine retina. Proc. Natl. Acad. Sci. USA. 89
Biotin labeling and hybridization of probe to chromosomes
Biotin labeling and hybridization of probe to chromosomes Suggested chemicals: Nick Translation Kit, Enzo Diagnostics Cat. No. 42803. Biotin labeling of plasmid or genomic DNA. Remove slides
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