Recombinant Human IL-4

Recombinant Human IL-4  (CX03)

产品说明(Description)

Recombinant Human Interleukin-4 is produced by our Mammalian expression system and the target gene encoding His25-Ser153 is expressed. 

Accession #: P05112

Known as: Interleukin-4; IL-4; B-Cell Stimulatory Factor 1; BSF-1; Binetrakin; Lymphocyte Stimulatory Factor 1; Pitrakinra; IL4

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.4.

 

质量控制(Quality Control) 

Purity:         Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin:  Less than 0.1 ng/ug (1 EU/ug) as determined by LAL test.

 

Bioactivity: Measured in a cell proliferation assay using TF-1 human erythroleukemic cells.

                     The ED50 for this effect is 0.01-0.05 ng/ml.

Bioactivity-BLI:
Loaded Cynomolgus IL-4RA-Fc(Cat#C939) on Protein A Biosensor, can bind  Human IL-4(Cat#CX03) with an affinity constant of 0.54 nM as determined in BLI assay.

 

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in distilled water.
Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.Reconstituted protein solution can be stored at 4-7°C for 2-7 days.Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Interleukin-4 (IL-4) is a pleiotropic cytokine that regulates diverse T and B cell responses including cell proliferation, survival and gene expression. IL-4 is produced by mast cells, T cells, and bone marrow stromal cells. IL-4 regulates the differentiation of naive CD4+ T cells into helper Th2 cells, characterized by their cytokine-secretion profile that includes secretion of IL-4, IL-5, IL-6, IL-10, and IL-13, which favor a humoral immune response. Another dominant function of IL-4 is the regulation of immunoglobulin class switching to the IgG1 and IgE isotypes. Excessive IL-4 production by Th2 cells has been associated with elevated IgE production and allergic response.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

M2-like macrophages exert hepatoprotection in acute-on-chronic liver failure through inhibiting necroptosis-S100A9-necroinflammation axis[J]. Li Bai. et al. Cell Death and Disease. 2021.

 

FOR RESEARCH USE ONLY