Recombinant Human/Mouse/Rat BM

Recombinant Human/Mouse/Rat BMP-2  (C012)

 

产品说明(Description)

Recombinant Human/Mouse/Rat Bone Morphogenetic Protein 2 is produced by our E.coli expression system and the target gene encoding Gln283-Arg396 is expressed. 

Accession #: P12643

Known as: Bone Morphogenetic Protein 2; BMP-2; Bone Morphogenetic Protein 2A; BMP-2A; BMP2; BMP2A

 

制剂(Formulation)

Lyophilized from a 0.2 μm filtered solution of 10mM HAc-NH4Ac, 4% D-Mannitol, pH 4.0.

 

质量控制(Quality Control) 

Purity:        Greater than 95% as determined by reducing SDS-PAGE.    

Endotoxin: Less than 0.1 ng/μg (1 EU/μg) as determined by LAL test.

 

复溶(Reconstitution)

Always centrifuge tubes before opening. Do not mix by vortex or pipetting.
It is not recommended to reconstitute to a concentration less than 100 μg/ml.
Dissolve the lyophilized protein in 50mM Acetic Acid.

Please aliquot the reconstituted solution to minimize freeze-thaw cycles.    

 

保存(Storage)

Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks.
Reconstituted protein solution can be stored at 4-7°C for 2-7 days.
Aliquots of reconstituted samples are stable at < -20°C for 3 months.     

 

背景(Background)

Bone Morphogenetic Protein-2 (BMP-2) is one of the bone-growth regulatory factors that belong to the transforming growth factor-beta (TGF-beta) superfamily of proteins. BMPs are synthesized as large precursor molecules, which are cleaved by proteolytic enzymes. The active form of BMP-2 can consist of a dimer of two identical proteins or a heterodimer of two related bone morphogenetic proteins.

 

电泳(SDS-PAGE)

 

参考文献(Reference)

PKC-δ deficiency in B cells displays osteopenia accompanied with upregulation of RANKL expression and osteoclast–osteoblast uncoupling[J]. Shangfu Li. et al. Cell Death and Disease. 2020.

Efficient catechol functionalization of biopolymeric hydrogels for effective multiscale bioadhesion[J]. KunyuZhang. et al. Materials Science and Engineering: C. 2019.

Biological activity of a nanofibrous barrier membrane containing bone morphogenetic protein formed by core-shell electrospinning as a sustained delivery vehiclet[J]. Huiyong Zhu. et al. J Biomed Mater Res. 2013.

miR-30 Family Members Negatively Regulate Osteoblast Differentiation[J]. Tingting Wu. et al. Journal of biological chemistry. 2012.

 

Reconstruction of comminuted long-bone fracture using CF/CPC scaffolds manufactured by rapid prototyping[J]. Sheng-Li Huang. et al. Med Sci Monit. 2012.

 

 

 

 

FOR RESEARCH USE ONLY