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- 文献和实验
- 技术资料
- 保存条件:
说明书
- 保质期:
说明书
- 英文名:
2×RealStar Probe Fast Mixture
- 库存:
大量
- 供应商:
上海康朗生物科技有限公司
- 规格:
ml/盒
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文献和实验Fast and reliable mini-prep RNA extraction from Neurospora crassa
otherwise. - Pulverize the mycelium in a mortar with liquid nitrogen - Transfer the powder into a mixture of 0.75 ml lysis buffer (0.6 M NaCl, 10 mM EDTA, 100 mM Tris HCl, pH 8.0, 4 SDS) and 0.75 ml phenol (saturated with 0.1 M Tris HCl, pH 8.0) in a 2 ml
Biotin labeling and hybridization of probe to chromosomes
times more than the probe DNA. Hybridization of probe to chromosomes. Denature the mixture by placing at 75 C for 5 min, immediately chilling on ice for 5 min, and spining down the solution. Place the slides (by using
DNA Probe Purification Protocol
microgram DNA due to the limiting nucleotide concentrations used. In this case, a carrier such as yeast tRNA should be added to Buffer DP to a final concentration of 2-5 ug/mL before use to ensure binding to HiBind resin. The mixture is stable at -70℃
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