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- 详细信息
- 文献和实验
- 技术资料
- 保质期：
12个月
- 英文名：
Exo-1
- 供应商：
上海汇谱仪器有限公司
- CAS号：
461681-88-9
- 规格：
10 mg
概述
- 产品名称
Exo-1 - 描述
Vesicular trafficking inhibitor - 生物学描述
Cell-permeable, reversible inhibitor of vesicular trafficking between endoplasmic reticulum and the Golgi apparatus. Inhibits exocytosis with an IC50 of approx. 20 μM. Rapidly releases Arf1 from Golgi membranes. Has similar mechanism of action to brefeldin A (ab120299). - 纯度
> 99%
性能
- 化学名称
Methyl 2-(4-fluorobenzamido)benzoate - 分子量
273.26 - 化学结构
- 分子式
C15H12FNO3 - CAS编号
461681-88-9 - PubChem识别号
310557 - 存放说明
Store at Room Temperature. The product can be stored for up to 12 months. - 溶解度概述
Soluble in DMSO to 100 mM and in ethanol to 25 mM - 处理
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour.
Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
- SMILES
O=C(Nc1ccccc1C(=O)OC)c2ccc(F)cc2 - 来源
Synthetic
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文献和实验The study of developmental mechanisms in postimplantation mammalian embryos can be performed for a short term using whole-embryo culture systems (see Chapters 7 and 8 ), or for an extended period using in utero (1 ,2 ) or exo utero
Exo Ovo Culture of Avian Embryos
Exo ovo culture of avian embryos is a technique for long-term culturing of embryos outside of their own shell and shell membranes. The problem of how to gain access to the avian embryo while allowing it to grow normally has been the subject
1. Cut DNA with 5'- and 3'- overhangs, gel check, phenol-Sevag extract and NaOAc/EtOH ppt. 2. Prepare one S1 nuclease tube (on ice) for each time point: 15ml S1 Buffer + 0.25U S1/ml (5U). 3. Use 5ml (=0.5mg) digested plasmid (in EB) per time
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