葡甘露聚糖检测试剂盒

UV-method for the determination of Glucomannan in plant
products, foodstuffs and other materials

Principle:
(β-mannanase)
(1) Ac-Glucomannan + H₂O → Ac-glucomanno-oligomers

(pH 12.5)
(2) Ac-Glucomanno-oligomers + H₂O → glucomanno-oligomers
+ acetate

(β-glucosidase + β-mannosidase)
(3) Glucomanno-oligomers + H₂O → D-glucose + D-mannose

(hexokinase)
(4) D-glucose + D-mannose + ATP → G-6-P + M-6-P + ADP

(glucose-6-phosphate dehydrogenase)
(5) G-6-P + NADP⁺ → gluconate-6-phosphate + NADPH + H⁺

(phosphomannose isomerase) (phosphoglucose isomerase)
(6) M-6-P → F-6-P → G-6-P

Kit size: 50 assays
Method: Spectrophotometric at 340 nm
Total assay time: 120 min
Detection limit: 1-100% of sample weight
Application examples:
Jelly sweets, cosmetics, food gums and other materials
Method recognition: Novel method