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- 详细信息
- 文献和实验
- 技术资料
- 保质期:
两年
- 英文名:
Safranine T
- 库存:
99
- 供应商:
江蓝纯
- CAS号:
477-73-6
属性
| 敏感性 | 对湿度敏感 |
| 溶解性 | Soluble in water (50 mg/ml at 20 °C), and alcohol. |
| 存贮条件 | 密封保存。 |
描述
| 别名 | 棉染红;盐基桃红;碱性红 2;藏红 Y 或 A;3,7-二氨基-2,8-二甲基-5-苯基酚嗪鎓氯化物;Safranin Y or A; Basic Red 2; Safranine O; 3,7-Diamino-2,8-dimethyl-5-phenylphenazinium chloride Cotton Red ; Gossypimine; C.I. 50240; |
| 应用 | 微量分析亚硝酸的试剂。氧化还原指示剂、酸碱指示剂、生物染色剂。 高纯级用在Flemming triple染色中的染色体染色;在吉姆萨染色中对革兰氏阴性细菌染色;在Safranine-fast green FCF染色中对植物细胞染色。 |
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文献和实验Coomassie Brilliant Blue G-250 in 50 ml 95% ethanol,add 100 ml 85% (w/v)phosphoric acid.Dilute to 1 liter when the dye has completely dissolved,and filter through Whatman #1 paper just before use.2.(Optional)1 M NaOH (to be used if samples are not readily
in 50 ml 95% ethanol, add 100 ml 85% (w/v) phosphoric acid. Dilute to 1 liter when the dye has completely dissolved, and filter through Whatman #1 paper just before use. (Optional) 1 M NaOH (to be used if samples are not readily soluble
Overgo Probing of High-Density Filters
digestion and contigs assembled. Hybridizations: Note: Done in a hybridization oven at 58℃. This temperature works well for overgos with GC content between 40% and 60%. Using overgos which are AT-rich may require lowering the hybridization temperature.
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