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- 保存条件:
常温
- 规格:
ml
缓冲液成分:0.01M PBS、0.5%GSA、N防腐剂
标记物浓度:以抗体计1mg/mL左右
使用建议:Flow-Cyt/ IF/IP:1:200-2000,IHC:1:500-2500,ELISA/WB:1:2000-20000,也可根据您实验的具体情况在此建议的稀释度范围之外合理使用。
储存方法:请避光存放在-20℃。
有效期限:请查看管体标签,请您在管体注明有效期内使用。
注意事项:虽然本试剂在有效期过后很长一段时间仍可以使用,但还是强烈建议您在管体规定的有效期内正确使用。请不要在脏乱和强光环境中取用,以免造成污染和失效。如果一次使用很少可以将其适当分装后使用,这样可以有效的防止试剂因为频繁使用而造成污染。另外,此抗体仅用于科研,不用于诊断。
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文献和实验Standard Protocols for the Construction of Fab Libraries
Fab libraries, in which light-chain (LC) and heavy-chain (HC) variableregion genes are cloned into a phagemid vector and subsequently displayed on the surface of the filamentous phage particle, have been widely used for the isolation
Construction of Large Nave Fab Libraries
In recent years, a number of single-pot antibody (Ab) libraries have been described, which permit the rapid isolation of high-affinity Abs against large panels of antigens (Ags). Na�ve libraries have been generated by tapping the natural
Selection of Human Fab Libraries by Phage Display
This protocol describes the selection of human antibody libraries in Fab format by phage display. It includes panning against immobilized antigens, biotinylated antigens in solution, and cell surface antigens.
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