Skeletal muscle regenerates by the proliferation of mononuclear myogenic precursor cells called myoblasts that ultimately fuse and become incorporated into multinucleated myotubes, which later mature into myofibres. This process occurs during the embryonic histogenesis of muscle and in postnatal muscle regenerating in response to injury, or in myopathies such as Duchenne muscular dystrophy. The fusion of myoblasts is specific to skeletal muscel. Myoblasts that do not form muscle fibers differentiate into satellite cells. Skeletal muscel myoblasts express FGF receptor and IGF expression is increased during myoblast differentiation in culture. The human skeletal muscle myoblast culture is a convenient in vitro model for the study of cellular development and differentiation, RI matablism and tissue repair.HSkMM from AcceGen Research Laboratories are isolated from human muscle of the pectoral girdle. HSkMM are cryopreserved on primary culture or passage one and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HSkMM are characterized by immunofluorescent method with antibodies to myosin, actin and actinin. HSkMM are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 骨骼肌的再生是通过单个核肌原体细胞(称为成肌细胞)的增殖实现的,这些成肌细胞最终融合并并入多核肌管,然后成熟为肌纤维。这一过程发生在肌肉的胚胎组织发生和出生后肌肉损伤后的再生过程中,或在杜氏肌营养不良等肌病中。成肌细胞的融合是骨骼肌特有的。不形成肌纤维的成肌细胞分化为卫星细胞。骨骼肌成肌细胞表达FGF受体,培养成肌细胞分化过程中IGF表达增加。人骨骼肌成肌细胞培养是研究细胞发育分化、RI稳定和组织修复的一种方便的体外模型。AcceGen研究实验室的HSkMM是从人的胸带肌肉中分离出来的。HSkMM在原代培养或传代培养中冷冻保存,冷冻交付。每个小瓶含有1ml体积的>5×10^5细胞。HSkMM通过免疫荧光法检测肌球蛋白、肌动蛋白和肌动蛋白抗体。HSkMM对HIV-1、HBV、HCV、支原体、细菌、酵母和真菌均为阴性。
Quality Control
All cells test negative for mycoplasma, bacteria, yeast, and fungi.