Human Nucleus Pulposus Cells人髓

Human Nucleus Pulposus Cells

ABC-TC3728

Human

1 vial

1

Dry Ice

Liquid Nitrogen

Nucleus pulposus is the jelly-like substance in the middle of the intervertebral disc. It functions to distribute hydraulic pressure in all directions within each disc under compressive loads. The nucleus pulposus consists of collagen fibrils, proteoglycan aggrecans and nucleus pulposus cells. The nucleus pulposus cells reside in an environment that has a limited vascular supply and generate energy through anaerobic glycolysis. The cells synthesize collagen II and X; express hypoxia-inducible factor-1, and secrete interleukins-1, -6, and -10 and granulocyte-macrophage colony-stimulating factor in culture. Mechanical stress on nucleus pulposus cells promotes cell proliferation. The nucleus pulposus cell culture provides an in vitro model for the study of cellular and molecular events involved in disc degeneration, tissue engineering and cell therapy for spine disc disorders.HNPC from AcceGen Research Laboratories are isolated from nucleus pulposus of human intervertibral disc. HNPC are cryopreserved at primary or passage one culture and delivered frozen. Each vial contains >5×10^5 cells in 1 ml volume. HNPC are characterized by immunofluorescent method with antibodies to fibronectin and vimentin. HNPC are negative for HIV-1, HBV, HCV, mycoplasma, bacteria, yeast and fungi. 
髓核是位于椎间盘中间的果冻状物质。它的作用是在压缩载荷下向每个圆盘内的所有方向分配液压压力。髓核由胶原原纤维、蛋白多糖凝集物和髓核细胞组成。髓核细胞驻留在一个血管供应有限的环境中，通过厌氧糖酵解产生能量。细胞合成胶原II和X；表达缺氧诱导因子-1，培养时分泌白细胞介素-1、-6、-10和粒细胞-巨噬细胞集落刺激因子。机械应力对髓核细胞增殖有促进作用。髓核细胞培养为研究椎间盘退变、组织工程和椎间盘疾病的细胞治疗过程中的细胞和分子事件提供了一个体外模型。HNPC来自AcceGen研究实验室，从人椎间盘髓核中分离得到。HNPC在原代或传代培养时冷冻保存，冷冻交付。每个小瓶含有1ml体积的>5×10^5细胞。利用纤维连接蛋白和波形蛋白抗体免疫荧光法对HNPC进行了表征。HNPC对HIV-1、HBV、HCV、支原体、细菌、酵母和真菌均呈阴性。

All cells test negative for mycoplasma, bacteria, yeast, and fungi.

For research use only

Cartilage Cells