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- 2026年02月01日
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| Source | E.coli |
| Description | Recombinant Human Tumor Necrosis Factor alpha is produced by our E.coli expression system and the target gene encoding Val77-Leu233 is expressed. |
| Names | Tumor Necrosis Factor, Cachectin, TNF-Alpha, Tumor Necrosis Factor Ligand Superfamily Member 2, TNF-a, TNF, TNFA, TNFSF2 |
| Accession # | P01375 |
| Formulation | Lyophilized from a 0.2 μm filtered solution of 20mM PB, 150mM NaCl, pH 7.0. |
| Shipping | The product is shipped at ambient temperature. |
| Reconstitution | Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 μg/ml. Dissolve the lyophilized protein in ddH2O. Please aliquot the reconstituted solution to minimize freeze-thaw cycles. |
| Storage | Lyophilized protein should be stored at < -20°C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 4-7°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months. |
| Biological Activity | ED50 is less than 0.03 ng/ml. Specific Activity of 3.0 x 10^7 IU/mg. |
| Purity | Greater than 95% as determined by reducing SDS-PAGE. |
| Endotoxin | Less than 0.1 ng/µg (1 IEU/µg) as determined by LAL test. |
| Amino Acid Sequence |
MVRSSSRTPSDKPVAHVVANPQAEGQLQWLNRRANALLANGVELRDNQLVVPSEGLYLIYSQVLF KGQGCPSTHVLLTHTISRIAVSYQTKVNLLSAIKSPCQRETPEGAEAKPWYEPIYLGGVFQLEKG DRLSAEINRPDYLDFAESGQVYFGIIAL
|
| Background | TNFα is a homotrimer with a subunit molecular mass of 17 kD and plays a major role in growth regulation, differentiation, inflammation, viral replication, tumorigenesis, autoimmune diseases and in viral, bacterial, fungal, and parasitic infections. Besides inducing hemorrhagic necrosis of tumors, TNF was found to be involved in tumorigenesis, tumor metastasis, viral replication, septic shock, fever, inflammation, and autoimmune diseases including Crohn’s disease, and rheumatoid arthritis as well as graft-versus-host disease. |
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文献和实验in their discovery. Recently, a nomenclature committee has adopted and promoted a simplified naming system; each member is designated tumor necrosis factor super family, TNFSF, with a numerical suffix. Thus, for example, TNF-α becomes TNFSF2 and Fas ligand
a look from the following website just by clicking "Tyrosine Phosphorylation and Dimerization" http://www.sciencedirect.com/science?_ob=ArticleURL&_udi=B6WSN-43F85YV-2&_user=1111158&_coverDate=06%2F29%2F2001&_rdoc=1&_fmt=full&_orig=search
扫描仪中，都采用机械式的二维X，Y线性扫描技术实现，即X，Y方向都采用直线驱动器和直线导轨实现往复运动。此类装置，由于驱动系统的频率限制，驱动器的扫描惯性大，使得扫描效率低，分析时间相当长；并且往复行程长，对直线导轨的精度要求相当高。二、光机结合的二维扫描系统为同样实现生物芯片的二维扫描，我们的实验装置设计如图2，采用了振镜和大数值孔径的远心f-è物镜相结合实现X方向扫描，Y方向的运动仍采用直线驱动器和直线导轨实现。 系统中，对于f-è物镜，满足x＝2fè（è为振镜的摆动角度，f为物镜焦距）的线性
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