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- 江蓝纯
- G111730
- 2025年07月07日
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- 文献和实验
- 技术资料
- 保质期：
两年
- 英文名：
Glycogen
- 库存：
99
- 供应商：
江蓝纯
- CAS号：
9005-79-2
属性
| 溶解性 | Solubility in water: soluble (17.7 g/100 ml) Solubility in other solvents: aq. solutions may appear hazy; sparingly in alcohol |
| 存贮条件 | 储存温度 2-8℃ |
描述
| 产品介绍 | Glycogen is an inert carrier which significantly increases the efficiency of extracted DNA and RNA by ethanol precipitation. It is an analogue of starch, has a similar structure to amylopectin, and functions as a secondary long-term energy storage. Glycogen is suitable as a carrier substance for nucleic acids precipitation. |
| 别名 | alpha-1,6-葡聚糖;D-(+)-GLYCOGEN |
| 应用 | An inert carrier which significantly increases the efficiency of extracted DNA and RNA by ethanol precipitation. |
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文献和实验，而且容易产生极化，（极化是糖原颗粒趋向于细胞的一端）。故用Gendre氏固定液效果较佳，其配法如下： 苦味酸饱和于95%酒精 85ml 40%甲醛 10ml 冰醋酸 5ml 3、各种试剂必须化学纯，亚硫酸钠须有浓厚气味，器皿必须洁净而干燥，染色缸亦是。 4、如果Schiff氏试剂，在经过活性碳吸附漂白后不显无色，首先应考虑试剂中的偏重亚硫酸钠是否失效。如果偏重亚硫酸钠气味不浓，使用时可考虑适当的增加用量。其次考虑碱性品红本身，常常虽属同一生产厂家，但不同批号，其效果
the glycogen will precipitate instantaneously. Collect the precipitate by centrifugation and air dry completely the pellet. Weigh the powder so obtained and resuspend it in autoclaved milli-Q water at a convenient stock concentration (10-20 mg/ml is suggested
James Hardwick CNBr Cleavage Procedure
yield. 4. Spin 1-5 min in the microfuge. Transfer supe. to a new microfuge tube. 5. Speed Vac dry (30min for 200 microliters). 6. Resuspend in 500 microliters of MQH2O and dry on the speed vac again (approx 2 hrs.). This should get rid
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