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文献和实验Southern Blot using Neutral Transfer
-Elaine Pinheiro and Aurora Burds Connor MIT, Jan2006 This protocol works very well with Hybond-XL membrane from GE Healthcare (formerly Amersham). 1) Digest your genomic DNA, using about 10 μg per reaction in 100 μl total digestion
Membrane Transfer and Crosslinking for RNA
does not allow passive transfer in efficient, quantitative, or reproducible yield. Thus, polyacrylamide gels should be transferred by electroblotting. This method has shown that a 32 P/biotinylated RNA probe is transferred at 100% efficiency to Ambion's
Freeze-Crack P granule Staining Protocol
Freeze-Crack P granule Staining Protocol 1. Prepare humidity chamber: line large square plate with humid paper towels and place 4 5ml plastic pipets across chamber to support slides. Place aluminum block in dry ice
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