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文献和实验实验助攻 | WB 实验 (Western Blot) 杂带太多?原因就这几个!
,并不会与重组 β-tubulin 蛋白发生交叉反应。 c-Myc 抗体 (YA497) www.medchemexpress.cn/antibody/c-myc-rabbit-mab.html 该抗体可检测 c-Myc 总蛋白的内源水平。 HRP-conjugated AffiniPure Goat Anti-Mouse IgG H&L www.medchemexpress.cn/antibody/peroxidase-conjugated-affinipure-goat-anti-mouse
and wash the blot 3 times, 5-7 minutes each, with 0.5% BSA in TBST.11. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection
each, with 0.5% BSA in TBST. Incubate with Goat anti-Rabbit/alkaline phosphatase (from Promega; 1:7500 inTBST with 5% BSA) for 1 hour at room temp with shaking. I have also used successfully Amersham ECL detection which employs goat anti
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