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文献和实验Principles and Methods 9. Gel Filtration, Principles and Methods 10. GST Gene Fusion System 11. Hydrophobic Interaction Chromatography, Principles and Methods 12. Ion Exchange Chromatography, Principles and Methods 13. Microarray Handbook 14.
2-D Polyacrylamide Gel Electrophoresis
for a few minutes to evaporate the remaining xylene. 13.Add 400 µl IEF buffer. 14.Vortex vigorously for 1 min. 15.Incubate for 5 min at RT. 16.Vortex vigorously for 1 min. 17.Spin sample down at 14,000 g for 5-10 min at room temp.•To lyse a 5-8 µm tissue
2 Dimensional Gel Electrophoretic Analysis for Chicken Egg
with its lid. 11. Harvest the rehydrated IPG strip. 12. Rinse the IPG strip to remove the mineral oil. 13. Place the IPG strip into the other strip tray ditches with the gel-surfaces face upward. 14. Add wicks soaked with buffer solution
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