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源叶现货试剂/标准品，库存足，周末发货Cytoscape 教程来了！快速实现顶刊同款通路网络图五大应用案例：Mustang Q 膜层析应用全解析1 个小工具，一次性搞定流程图、质粒图谱和信号通路图- 详细信息
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文献和实验Protocol for Co-IP of different proteins with RFP-MeCP2 Preperation of cells (for p100): • 1st day split 293T EBNA cells in DMEM (high glucose + 10% FCS + 5mM glutamine + 5µg/ml gentamycine) • 2nd day cells
into eppyincubate 45' 4℃ à Vortex several times duringspin 10 secs 4℃divide sample: ~400 l for IP/ ~ 100 l for WesternIP -- 1° Ab => 4℃ 1 hour rockingif 1° is not rab.à 2° Ab => 4℃ 1 hour rocking (RaM 2l)-- PrA/Seph 4℃ 1 hour rocking 40l-- Spin down 4℃ 1'-- Wash 3X
is the same for all species, there are some differences in handling cells. This protocol is developed and tested for E. coli . It should work the same way for other bacteria but that remains to be proven. Published protocols also exist for other bacterial species, including
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