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文献和实验96 Well Mating Protocol platewise
a recipient dilution of 1:100. (1ul)(96 wells)(14 96well plates) = 1.34 mls into 138.66 mls LB Rhamnose - If your recipient OD is 0.800, you would make recipient dilution of 1:50. (2ul)(96 wells)(14 96wellplates) = 2.68 mls into 137.32 mls LB Rhamnose
96 Well Mating Protocol platewise
Chemicals needed:Glucose L-(+)-Arabinose Sigma Cat # A3256 L-Rhamnose Sigma cat # R3875 Cb (carbenicillin) Sigma cat # 1389DL-p-Chlorophenylalanine Sigma cat # C6506 Media Recipes: YEG recombination plates:1 liter Milli Q water5 g yeast
Standard Operating Procedures for T1-Phage Testing Assay
, Rossville 50 ml polypropylene tubes, Falcon, Corning Q-tray, Genetix 96-pins stamping tool, V & P Scientific, Inc. Autoclave, refrigerator, incubator, waterbath, and laminar flow hood. III. Procedures: Method
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