SUPERDEX 200 PREP GRADE 150 ML

BAC DNA Isolation from 200 ml Cultures by a Cleared Lysate Method

antibiotic. After incubating at 37 degrees C for 8-10 hours with 250 rpm shaking, the culture is transferred to a 500 ml flask containing 200 ml of the same medium and incubated for 8-10 hours under the same conditions. After harvesting the cells

A quick RNA mini-prep for Neurospora mycelial cultures

is as follows: 1. Conidia from slants (grown in 16 x 150 mm test tubes containing 8 ml of solid medium) are resuspended in 50 ml of Horowitz complete medium (Horowitz 1947 J. Biol. Chem. 171:255-262) and the cultures grown overnight with shaking at 30°C

BACDNAIsolationfrom200mlCulturesbyaClearedLysateMethod

per 200 ml of original cell growth is approximately 150 ug.