柱式法总RNA-vRD II抽提纯化试剂盒（Ribospin

Cat.No.	Product Description	Size
322-108	RibospinTM vRDⅡ	8
322-150	RibospinTM vRDⅡ	50

 



Features and Benefits
 
- Spin column format
- Stable and consistent yield
- Preparation time : ~ 15 minutes
- No phenol / chloroform extraction
- No ethanol precipitation
- Micro column & carrier RNA enhance the performance of viral sample extraction
- Various viral samples : cell-free fluid, cell-culture supernatant, plasma, serum, swab, urine and virus-infected samples
- Ready for use in PCR, RT-PCR, real-time PCR and other analytical procedures
 



Description
 
Ribospin^TM vRD II provides a convenient method for isolation of RNA and DNA from cell-free fluid, cell-cultrue supernatant, plasma, serum, swab, urine, and virusinfected samples.
Ribospin^TM vRD II procedures employed the glassfiber membrane technology for the fastest and the most convenient of high purity RNA and DNA isolation, instead of conventional alcohol  recipitation or phenol/chloroform extraction. Ribospin ^TM vRD II buffer system provides the effective binding condition of RNA and DNA to glassfiber membrane and the impurities on the membrane are washed away by two different wash buffers. At last, pure RNA and DNA are eluted by nuclease-free water. Whole procedure takes only 15 minutes and the purified nucleic acid is suitable for PCR, RT-PCR, or any downstream application without further manipulation.
Ribospin^TM vRD II procedure should be performed at room temperature. The purified nucleic acid should be treated with care because RNA is very sensitive to contaminants, such as RNases, often found on general labware and dust. To ensure RNA-stability, it is recommended to store at 4 °C for immediate analysis or to freeze at -70 °C for long-term storage.
 



Procedure
 




Specification