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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
在-20℃ to -80℃
- 保质期:
12个月
- 英文名:
Recombinant Human SerpinE1 / PAI-1 Protein (His Tag)
- 库存:
99
- 供应商:
北京义翘神州科技股份有限公司
- 规格:
50 µg/1 mg
| 规格: | 50 µg | 产品价格: | ¥3870.0 |
|---|---|---|---|
| 规格: | 1 mg | 产品价格: | ¥29480.0 |
蛋白名称:Human SerpinE1 / PAI-1 Protein (His Tag)
蛋白构建:A DNA sequence encoding the human SerpinE1 precursor (NP_000593.1) (Met 1-Pro 402) was expressed with a C-terminal polyhistidine tag.
表达宿主:HEK293 Cells
蛋白纯度:> 97 % as determined by SDS-PAGE
蛋白活性:Measured by its ability to inhibit uPA cleavage of a peptide substrate, N-carbobenzyloxy-Gly-Gly-Arg-7-amido-4-methylcoumarin (Z-GGR-AMC). The IC50 value is < 60 nM.
蛋白内毒素:< 1.0 EU per μg of the protein as determined by the LAL method
预测N端:Val 24
蛋白分子量:The mature recombinant human SerpinE1 consists of 390 amino acids and predicts a molecular mass of 44.2 kDa. In SDS-PAGE under reducing conditions, the apparent molecular mass of rhSerpinE1 is approximately 45 kDa.
蛋白NP号:NP_000593.1
蛋白氨基酸序列:Met1-Pro402
蛋白标签:C-His
蛋白保存条件:Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验2, Reichel, CA; et al. Components of the Plasminogen Activation System Promote Engraftment of Porous Polyethylene Biomaterial via Common and Distinct Effects. PLoS ONE, Pubmed: 25658820
3, 王迪. et al. PAI-1 过表达促进食管鳞癌细胞的侵袭和迁移. 遗传
Strategies to Optimize Protein Expression in E. coli
Peng, L., Xu, Z.N., Fang, X.M., Wang, F., Yang, S., and Cen, P.L. 2004. Preferential codons enhancing the expression level of human beta‐defensin‐2 in recombinant Escherichia coli. Protein Pept. Lett. 11:339
Identifying Protein Interactions by Hydroxyl‐Radical Protein Footprinting
Basic Protocol 1: Generation of an Active Recombinant Protein That is End‐Labeled Basic Protocol 2: Hydroxyl Radical Cleavage of Protein in Absence and Presence of Ligand Basic Protocol 3: Analyze Gel
Purification of Human Multiprotein Complexes using OneSTrEP Technology (PROT41)
complexes from human HeLa S3 cells in a scale and purity optimized for characterization by mass spectrometry. For this purpose, we use stably expressed One-STrEP -tag ® fusion proteins. This approach was successfully used in characterization of histone
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