- ￥1270 - 4520
- Sino Biological
- 北京
- 11684-V08H
- 2026年01月26日
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- 详细信息
- 询价记录
- 文献和实验
- 技术资料
- 保存条件：
-20℃ to -80℃
- 保质期：
12个月
- 英文名：
Recombinant H1N1 (A/Puerto Rico/8/1934) HA Protein (ECD,His Tag)
- 库存：
99
- 供应商：
北京义翘神州科技股份有限公司
- 规格：
10.00 µg/50.00 µg/100.00 µg
| 规格： | 10.00 µg | 产品价格： | ￥1270.0 |
|---|---|---|---|
| 规格： | 50.00 µg | 产品价格： | ￥2440.0 |
| 规格： | 100.00 µg | 产品价格： | ￥4520.0 |
H1N1 Hemagglutinin/HA蛋白|Hemagglutinin/HA protein|Hemagglutinin/HA(H1N1, C-His)（产品说明）
蛋白名称：Hemagglutinin/HA蛋白, Hemagglutinin/HA protein
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Gln 528) of the influenza A hemagglutinin (A/Puerto Rico/8/1934 (H1N1)) (ABD77675.1), was expressed, fused with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 85 % as determined by SDS-PAGE.
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Asp 18
蛋白分子量：The secreted recombinant influenza A H1N1 (A/Puerto Rico/8/1934 (H1N1)) HA comprises 522 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：ABD77675.1
蛋白氨基酸序列：Met1-Gln528
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
蛋白名称：Hemagglutinin/HA蛋白, Hemagglutinin/HA protein
蛋白构建：A DNA sequence encoding the extracellular domain (Met 1-Gln 528) of the influenza A hemagglutinin (A/Puerto Rico/8/1934 (H1N1)) (ABD77675.1), was expressed, fused with a C-terminal polyhistidine tag.
表达宿主：HEK293 Cells
蛋白纯度：> 85 % as determined by SDS-PAGE.
蛋白活性：0
蛋白内毒素：< 1.0 EU per μg of the protein as determined by the LAL method
预测N端：Asp 18
蛋白分子量：The secreted recombinant influenza A H1N1 (A/Puerto Rico/8/1934 (H1N1)) HA comprises 522 amino acids and has a predicted molecular mass of 59 kDa. As a result of glycosylation, it migrates as an approximately 60-70 kDa band in SDS-PAGE under reducing conditions.
蛋白NP号：ABD77675.1
蛋白氨基酸序列：Met1-Gln528
蛋白标签：C-His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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- 作者
- 内容
- 询问日期
文献和实验该产品被引用文献
1, Dai, J; et al. Molecular adjuvant Ag85A enhances protection against influenza A virus in mice following DNA vaccination. Viruses, PMID: 23223215
2, Wu, CY; et al. A VLP vaccine induces broad-spectrum cross-protective antibody immunity against H5N1 and H1N1 subtypes of influenza A virus. PloS one, PMID: 22879951
3, Gupta, N; et al. Whole-Virus Screening to Develop Synbodies for the Influenza Virus. Bioconjugate chemistry, PMID: 27658460
4, Pardi, N; et al. Nucleoside-modified mRNA vaccines induce potent T follicular helper and germinal center B cell responses. The Journal of experimental medicine, PMID: 29739835
5, Luganini, A; et al. The Cranberry Extract Oximacro® Exerts in vitro Virucidal Activity Against Influenza Virus by Interfering With Hemagglutinin. Frontiers in microbiology, PMID: 30131793
6, Andersen, TK; et al. Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules. NPJ vaccines, PMID: 30775000
7, Grodeland, G; et al. Targeting of HA to chemokine receptors induces strong and cross-reactive T cell responses after DNA vaccination in pigs. Vaccine, PMID: 31836256
8, Grødeland, G; et al. Induction of Cross-Reactive and Protective Antibody Responses After DNA Vaccination With MHCII-Targeted Stem Domain From Influenza Hemagglutinin. Frontiers in immunology, PMID: 32269566
9, Wang, M; et al. Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice. Journal of visualized experiments : JoVE, PMID: 32658200
10, Szodoray, P; et al. Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity. Cell reports, PMID: 34380042
11, Borriello, F; et al. An adjuvant strategy enabled by modulation of the physical properties of microbial ligands expands antigen immunogenicity. Cell, PMID: 35148840 1
2, Gregoire, C; et al. Viral infection engenders bona fide and bystander subsets of lung-resident memory B cells through a permissive mechanism. Immunity, PMID: 35768001 1
3, Olukitibi, TA; et al. Development and characterization of influenza M2 ectodomain and/or hemagglutinin stalk-based dendritic cell-targeting vaccines. Frontiers in microbiology, PMID: 36003947 1
4, Wang, B; et al. Wearable bioelectronic masks for wireless detection of respiratory infectious diseases by gaseous media. Matter, PMID: 36157685 1
5, Tjärnhage, E; et al. Trimeric, APC-Targeted Subunit Vaccines Protect Mice against Seasonal and Pandemic Influenza. Journal of virology, PMID: 36719241 1
6, Li, X; et al. Prevention and treatment of HPV-related cancer through a mRNA vaccine expressing APC-targeting antigen. Immunology, PMID: 38471664 1
7, Wu, M; et al. Innervation of nociceptor neurons in the spleen promotes germinal center responses and humoral immunity. Cell, PMID: 38772371 1
8, Ko, KH; et al. A vaccine platform targeting lung-resident memory CD4+ T-cells provides protection against heterosubtypic influenza infections in mice and ferrets. Nature communications, PMID: 39609429 1
9, Cardon, A; et al. Single cell profiling of circulating autoreactive CD4 T cells from patients with autoimmune liver diseases suggests tissue imprinting. Nature communications, PMID: 39880819
20, Agrafiotis, A; et al. Clonally expanded IgG, but not IgA, antibody-secreting cells preferentially target influenza nucleoprotein following homologous and heterologous infections. Journal of immunology (Baltimore, Md. : 1950), PMID: 40713886
2, Wu, CY; et al. A VLP vaccine induces broad-spectrum cross-protective antibody immunity against H5N1 and H1N1 subtypes of influenza A virus. PloS one, PMID: 22879951
3, Gupta, N; et al. Whole-Virus Screening to Develop Synbodies for the Influenza Virus. Bioconjugate chemistry, PMID: 27658460
4, Pardi, N; et al. Nucleoside-modified mRNA vaccines induce potent T follicular helper and germinal center B cell responses. The Journal of experimental medicine, PMID: 29739835
5, Luganini, A; et al. The Cranberry Extract Oximacro® Exerts in vitro Virucidal Activity Against Influenza Virus by Interfering With Hemagglutinin. Frontiers in microbiology, PMID: 30131793
6, Andersen, TK; et al. Enhanced germinal center reaction by targeting vaccine antigen to major histocompatibility complex class II molecules. NPJ vaccines, PMID: 30775000
7, Grodeland, G; et al. Targeting of HA to chemokine receptors induces strong and cross-reactive T cell responses after DNA vaccination in pigs. Vaccine, PMID: 31836256
8, Grødeland, G; et al. Induction of Cross-Reactive and Protective Antibody Responses After DNA Vaccination With MHCII-Targeted Stem Domain From Influenza Hemagglutinin. Frontiers in immunology, PMID: 32269566
9, Wang, M; et al. Evaluation of T Follicular Helper Cells and Germinal Center Response During Influenza A Virus Infection in Mice. Journal of visualized experiments : JoVE, PMID: 32658200
10, Szodoray, P; et al. Integration of T helper and BCR signals governs enhanced plasma cell differentiation of memory B cells by regulation of CD45 phosphatase activity. Cell reports, PMID: 34380042
11, Borriello, F; et al. An adjuvant strategy enabled by modulation of the physical properties of microbial ligands expands antigen immunogenicity. Cell, PMID: 35148840 1
2, Gregoire, C; et al. Viral infection engenders bona fide and bystander subsets of lung-resident memory B cells through a permissive mechanism. Immunity, PMID: 35768001 1
3, Olukitibi, TA; et al. Development and characterization of influenza M2 ectodomain and/or hemagglutinin stalk-based dendritic cell-targeting vaccines. Frontiers in microbiology, PMID: 36003947 1
4, Wang, B; et al. Wearable bioelectronic masks for wireless detection of respiratory infectious diseases by gaseous media. Matter, PMID: 36157685 1
5, Tjärnhage, E; et al. Trimeric, APC-Targeted Subunit Vaccines Protect Mice against Seasonal and Pandemic Influenza. Journal of virology, PMID: 36719241 1
6, Li, X; et al. Prevention and treatment of HPV-related cancer through a mRNA vaccine expressing APC-targeting antigen. Immunology, PMID: 38471664 1
7, Wu, M; et al. Innervation of nociceptor neurons in the spleen promotes germinal center responses and humoral immunity. Cell, PMID: 38772371 1
8, Ko, KH; et al. A vaccine platform targeting lung-resident memory CD4+ T-cells provides protection against heterosubtypic influenza infections in mice and ferrets. Nature communications, PMID: 39609429 1
9, Cardon, A; et al. Single cell profiling of circulating autoreactive CD4 T cells from patients with autoimmune liver diseases suggests tissue imprinting. Nature communications, PMID: 39880819
20, Agrafiotis, A; et al. Clonally expanded IgG, but not IgA, antibody-secreting cells preferentially target influenza nucleoprotein following homologous and heterologous infections. Journal of immunology (Baltimore, Md. : 1950), PMID: 40713886
相关实验
His 标签蛋白纯化效果不理想，宝宝心里苦呀。今天，小编来跟大家一起找找原因。 纯化所得组分中没有收集到重组的His标签蛋白。该问题主要可以分为以下两个方面： 1、His标签蛋白没有结合到填料上就流穿了 原因一 超声的功率不对。超声功率过高，容易导致蛋白炭化；功率过低，蛋白释放不出来。 建议 改变超声功率，同时可以在超声前加入溶菌酶。 原因二 结合缓冲液条件不合适。 建议 检查结合缓冲液中是否有影响结合的因素，如：金属离子螯合剂、强还原剂、过高的咪唑浓度。同时，优化缓冲液的pH、盐
蛋白磷酸化就在有丝分裂、细胞死亡、DNA 损伤修复、DNA 复制和重组过程中发挥着直接的作用。 组蛋白翻译后修饰多发生在组蛋白的 N-端尾部，包括甲基化、乙酰化、磷酸化、ADP-核糖基化、泛素化和小分子泛素化修饰，这些修饰有助于其他蛋白质与 DNA 的结合，从而产生协同或者拮抗作用来调控基因转录。例如，乙酰化使组蛋白尾部正电荷减少，从而削弱了与带负电荷 DNA 骨架的作用，而促进染色质呈开放状态, 甲基化激活或抑制基因功能主要依赖于修饰的位点，主要与赖氨酸残基的单甲基化、双甲基化或三甲基化有关。 组蛋白修饰最基本
重组蛋白纯化基本原则蛋白的分离纯化是生物工程下游阶段一个比较重要的部分，尤其在基因工程重组蛋白的分离纯化中，上游过程的许多因素会直接影响到下游蛋白的分离，充分利用上游对下游的影响，对蛋白的纯化做一个全面的考虑和整体的设计。是否带有亲和标签，His标签，GST标签等，不同的亲和标签选择不同的纯化方案；可能是可溶性表达，可能形成包涵体，可溶性的蛋白往往需要复杂的纯化步骤，而包涵体易于分离，纯度较高，但回收具有生物活性的蛋白却变的相当困难，需要对聚集的蛋白进行变复性，通常活性蛋白的得率比较低；是否
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重组H1N1 (A/Puerto Rico/8/1934) HA蛋白 (ECD,His标签)
￥1270 - 4520
