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- Sino Biological
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- 2025年08月12日
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50 µg
蛋白名称：SUV420H2蛋白, SUV420H2 protein
蛋白构建：A DNA sequence encoding the amino acid sequence (Gly 2-Leu 280) of human SUV420H2 (NP_116090.2) was fused with the N-terminal polyhistidine-tagged GST tag at the N-terminus.
表达宿主：E. coli
蛋白纯度：> 80 % as determined by SDS-PAGE
蛋白活性：0
蛋白内毒素：Please contact us for more information.
预测N端：Met
蛋白分子量：The recombinant human SUV420H2/GST chimera consists of 516 amino acids and has a calculated molecular mass of 60 kDa as estimated in SDS-PAGE under reducing conditions.
蛋白NP号：NP_116090.2
蛋白氨基酸序列：Gly2-Leu280
蛋白标签：N-GST & His
蛋白保存条件：Store it under sterile conditions at -20℃ to -80℃. It is recommended that the protein be aliquoted for optimal storage. Avoid repeated freeze-thaw cycles.
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文献和实验Small Scale GST-Tag Purification Under Nature Conditions
Small Scale GST-Tag Purification Under Nature Conditions According to AMERSHAM-BIOSCIENCES-GST fusion protein Handbook (pdf) Aliquot of Cell Pellet after Induction The idea is to aliquot cells after induction
GST fusion protein purification--GST融合蛋白纯化
GST fusion protein purification GST融合蛋白纯化John Mundy,Institute of Molecular Biology,Copenhagen,Denmark1)grow 20ml cells O/N37℃ dilute 50X into prewarmed LB,grow to 0.6 OD or about1hr.Induce w/ 2mM IPTG (238mg/0.5l),grow 3hr,spin 6k GS3 10',freeze
GST Fusion Protein Preparation
CLEAVAGEAlternate Mini PrepThaw out 1 ml aliquot of fusion protein lysate.Centrifuge to remove precipitate.Incubate 1 ml lysate with 0.4 ml 50% GST beads.Incubate at 4℃ with mixing for 1 hour.Centrifuge 5K 2' in 1.5 ml eppendorf tubes.Wash the beads 4X with PBST
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