- 询价
- BIO-RAD
- 4415-0307
- 2025年11月20日
- E, P, WB
- Mouse
- Viral
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- 详细信息
- 文献和实验
- 技术资料
- 免疫原：
FELINE LEUKAEMIA VIRUS p27
- 亚型：
IgG1
- 形态：
Liquid
- 保存条件：
-20°C 分装保存
- 克隆性：
Monoclonal Antibody
- 标记物：
Purified
- 适应物种：
Viral
- 保质期：
12 months from date of despatch
- 目录编号：
PF12J-10A
- 供应商：
伯乐生命医学产品（上海）有限公司
- 宿主：
Mouse
- 应用范围：
E, P, WB
- 浓度：
IgG concentration 1.0mg/ml
- 抗体英文名：
MOUSE ANTI FELINE LEUKAEMIA VIRUS p27
- 抗体名：
Feline Leukaemia Virus p27 antibody | PF12J-10A
- 规格：
0.25 mg
Mouse anti Feline Leukaemia Virus p27 antibody, clone PF12J-10A recognizes feline leukaemia virus (FeLV) p27 core protein (p27).
The oncoretrovirus FeLV is responsible for both neoplastic and non-neoplastic conditions, often resulting in a poor prognosis and fatal outcome for cats persistently infected. During persistent infection the virus, soluble viral antigens and antigen containing white blood cells are continuously present in the blood and diagnosis is mainly based upon detection of the p27 antigen - using ELISA to test for p27 as it circulates free in the bloodstream, and Immunofluorescence to detect p27 within the infected white cells.
储存信息
Avoid repeated freezing and thawing as this may denature the antibody. Storage in frost-free freezers is not recommended.
应用
This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications from the originators. Please refer to references indicated for further information. For general protocol recommendations, please visit the antibody protocols page.
| Application Name | Verified | Min Dilution | Max Dilution |
|---|---|---|---|
| ELISA | |||
| Immunohistology - Paraffin | √ | 1/100 | |
| Western Blotting | √ | 1/1,500 |
- Western Blotting
- MCA2551 detects a band of approximately 28kDa under reducing conditions. A strong band of 80kDa representing glycosylated FeLV gag gene product may also be present.
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文献和实验- Kipar, A. et al. (2000) Expression of viral proteins in feline leukemia virus-associated enteritis.
Vet Pathol. 37 (2): 129-36. - Kipar, A. et al. (2001) Comparative examination of cats with feline leukemia virus-associated enteritis and other relevant forms of feline enteritis.
Vet Pathol. 38 (4): 359-71. - Kipar, A. et al. (1998) Fatal enteritis associated with coronavirus infection in cats.
J Comp Pathol. 119 (1): 1-14.
The Mouse as Model System to Study Host‐Pathogen Interactions in Influenza A Infections
Figure 5. Immunohistochemical staining for viral proteins in infected lungs. C57BL/6J mice were infected intranasally with 2 × 103 FFU of PR8M virus. Serial lung sections were stained with anti‐influenza antibody
The production of monoclonal antibody by hybridoma
and grow up for ascites/ large flask production and further freeze down. Troubleshooting I strongly reccommend to use Southern Biotech Goat anti-Mouse Ig (H+L
Propagation, Quantification, Detection, and Storage of West Nile Virus
anti‐WNV E16 MAb (Oliphant et al., ) Secondary antibody, goat anti‐mouse horseradish‐peroxidase conjugated (Sigma, cat. no. 8924)
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