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- 详细信息
- 技术资料
- 保存条件：
负20度
- 保质期：
12个月
- 库存：
100
- 供应商：
柯雷生物
- 规格：
50ug
[ PROPERTIES ]
Residues: Pro400~Ile543 (Accession # Q9Y251), with N-terminal
His-Tag. Host: E. coli Subcellular
Location: Lysosome membrane; Peripheral membrane protein. Secreted. Nucleus.
Purity: >98%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. Predicted isoelectric point: 9.9 Predicted Molecular Mass: 17.9kDa Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months. Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed.
(Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.)
The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF -P LPDYWLSLLF KKLVGTKVLM ASVQGSKRRK LRVYLHCTNT DNPRYKEGDL TLYAINLHNV TKYLRLPYPF SNKQVDKYLL RPLGPHGLLS KSVQLNGLTL KMVDDQTLPP LMEKPLRPGS SLGLPAFSYS FFVIRNAKVAACI
[ REFERENCES ]
1. Kussie P.H., et al. (1999) Biochem. Biophys. Res. Commun. 261:183-187.
2. Toyoshima M., Nakajima M. (1999) J. Biol. Chem. 274:24153-24160.
3. Vlodavsky I., et al. (1999) Nat. Med. 5:793-802.
4. Dempsey L.A., et al. (2000) Glycobiology 10:467-475.
Residues: Pro400~Ile543 (Accession # Q9Y251), with N-terminal
His-Tag. Host: E. coli Subcellular
Location: Lysosome membrane; Peripheral membrane protein. Secreted. Nucleus.
Purity: >98%
Endotoxin Level: <1.0EU per 1μg (determined by the LAL method). Formulation: Supplied as lyophilized form in 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT, 0.01% sarcosyl, 5% trehalose, and preservative. Predicted isoelectric point: 9.9 Predicted Molecular Mass: 17.9kDa Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles. Store at 2-8oC for one month. Aliquot and store at -80oC for 12 months. Stability Test: The thermal stability is described by the loss rate of the target protein. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37oC for 48h, and no obvious degradation and precipitation were observed.
(Referring from China Biological Products Standard, which was calculated by the Arrhenius equation.)
The loss of this protein is less than 5% within the expiration date under appropriate storage condition.
[ SEQUENCES ]
The target protein is fused with N-terminal His-Tag, its sequence is listed below.
MGHHHHHHSGSEF -P LPDYWLSLLF KKLVGTKVLM ASVQGSKRRK LRVYLHCTNT DNPRYKEGDL TLYAINLHNV TKYLRLPYPF SNKQVDKYLL RPLGPHGLLS KSVQLNGLTL KMVDDQTLPP LMEKPLRPGS SLGLPAFSYS FFVIRNAKVAACI
[ REFERENCES ]
1. Kussie P.H., et al. (1999) Biochem. Biophys. Res. Commun. 261:183-187.
2. Toyoshima M., Nakajima M. (1999) J. Biol. Chem. 274:24153-24160.
3. Vlodavsky I., et al. (1999) Nat. Med. 5:793-802.
4. Dempsey L.A., et al. (2000) Glycobiology 10:467-475.
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