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- 详细信息
- 文献和实验
- 技术资料
- 供应商:
PeproTech(派普泰克)
- 库存:
大量
- 英文名:
Recombinant Rat IL-13
| Synonyms : |
NC300 (Human) P600 (Murine) |
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| Description : |
IL-13 is an immunoregulatory cytokine produced primarily by activated Th2 cells, and also by mast cells and NK cells. Targeted deletion of IL-13 in mice resulted in impaired Th2 cell development and indicated an important role for IL-13 in the expulsion of gastrointestinal parasites. IL-13 exerts anti-inflammatory effects on monocytes and macrophages and it inhibits the expression of inflammatory cytokines such as IL-1beta, TNF-alpha, IL-6 and IL-8. IL-13 has also been shown to enhance B cell proliferation and to induce isotype switching resulting in increased production of IgE. Blocking of IL-13 activity inhibits the pathophysiology of asthma. Human and murine IL-13 is cross-species reactive. Recombinant rat IL-13 is an 11.9 kDa protein consisting of 109 amino acid residues. |
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| Catalog #: |
400-16 |
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| Source : |
E.coli |
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| Purity : |
Greater than 98% by SDS-PAGE gel and HPLC analyses. |
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| Endotoxin Level : |
Endotoxin level is less than 0.1 ng per µg (1EU/µg). |
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| Biological Activity : |
The ED 50 as determined by the dose-dependant stimulation of the proliferation of human TF-1 cells is < 40 ng/ml, corresponding to a specific activity of > 2.5 x 104 units/mg. |
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| Selected References : |
Selected List of References Citing This Product |
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| AA Sequence : |
VRRSTSPPVA LRELIEELSN ITQDQKTSLC NSSIVWSVDI TAGGFCAALE SLTNISSCNA IHRTQRILNG LCNQKASDVA SSPPDTKIEV AQFISKLLNY SKQLFRYGH |
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| PubMed Link : |
IL-13+AND+rat |
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| Country of Origin : |
USA |
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文献和实验为纵坐标,在坐标纸上作图,画出标准曲线。 3. 根据样品 OD 值在该曲线图上查出相应 IL-13 含量。 试剂盒性能 1. 灵敏度 : 最小的 IL-13 检测浓度小于 15pg/ml 。 2. 特异性: 可同时检测重组或天然的大鼠 IL-13 。 不与 大鼠其它细胞因子有交叉反应。 3. 重复性:板内、板见变异系数均小于10.6 %。 注意事项
ListeningRain 本人欲买猪重组细胞因子白介素6(IL-6)和肿瘤细胞坏死因子(TNF-a),在网上搜集以及和试剂公司联系了很久,发现只有以色列的Prospec的规格比较适合!RD公司的只有大包装25ug(3000元左右)(太贵,而且我用的量比较少),Prospec有2ug和5ug,价格在600元左右,容易接受。 我是要在体外细胞实验中加入这两种细胞因子,作为干预措施,观察细胞对它们的反应性! 不知,有用过这家公司细胞因子的丁友
枪的枪头轻吹几下,即可使细胞因子或重组蛋白完全溶解。 对于不易溶解的细胞因子或蛋白,PeproTech会在说明书中进行备注(Note)。如在重组人IL-13(产品编号:200-13)的说明书中您会看到:Slow to dissolve (缓慢溶解),在重组人IL-11(产品编号:200-11)的说明中会看到: This solution is slow to dissolve.(该溶液溶解缓慢)。这种情况下,您最好能将要溶解的细胞因子或重组蛋白放在水平摇床(shaker)上低速摇一段
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