
01917 pNPP Substrate, 120 mL
- 询价
- STEMCELL™
- 加拿大
- 01917
- 2025年07月09日
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- 文献和实验
- 技术资料
- 库存:
666
- 供应商:
杭州百通生物技术有限公司
- 规格:
120 mL
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文献和实验DETECTION OF RECOMBINANT PROTEINS BY ELISA
2CO 3 slowly while stirring. Adjust pH to 8.6 and QS to 100 ml with H 2O. Substrate Solution (1 mg/ml paranitrophenyl phosphate PNPP) 100 mg capsule of PNPP (Sigma 104-100) 100 ml substrate buffer
procedures utilize substrates that produce soluble products. Ideally the enzyme substrates should be stable, safe and inexpensive. Popular enzymes are those which convert a colorless substrate to a colored product, e.g. p-nitrophenylphosphate (pNPP
Introduction to Antibodies - Enzyme-Linked Immunosorbent Assay (ELISA)
to the matrix, through the use of a colorimetric substrate. Major advantages of this technique are that the antigen does not need to be purified prior to use, and that these assays are very specific. However, one disadvantage is that not all antibodies
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