GFP-LC3 Reporter Autophagy Assay Kits include one immortalized GFP-LC3 reporter cell line to measure and track the levels of LC3 within the cell. The autophagy detection reagents and cell line have been optimized together to ensure the ability to measure and discriminate between cytosolic and lipidated LC3 to accurately measure the autophagic process. Sufficient enabling reagents are provided to perform 100 tests. Detailed assay instructions are included to assist in analysis.