- ¥800 - 1380
- HZbscience
- 中国/美国/德国
- HZ0161
- 2025年07月12日
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- 详细信息
- 技术资料
- 保存条件：
-20℃
- 保质期：
半年
- 英文名：
pCDNA3.1(-)/Myc-His A
- 库存：
200
- 供应商：
沪震生物
- 规格：
5μl
pCDNA3.1(-)/Myc-His A
产品信息
| 产品货号 | 产品名称 | 产品规格 | 优惠价 |
|---|---|---|---|
| HZ0161 | pCDNA3.1(-)/Myc-His A | 20μl |
¥请询价 |
使用说明
沪震质粒平台的各批次质粒菌株发货前均经过严格的多重验证，如存在质量问题，请在收到产品的三个月内通知我司。收到质粒后请短暂离心，取2μl转化至对应感受态中，挑取单克隆重新提取质粒后使用。
基本信息
| 启动子: | CMV |
|---|---|
| 复制子: | pUC ori，F1 ori |
| 终止子: | BGH poly（A）signal |
| 质粒分类: | 哺乳细胞载体;蛋白过表达质粒 |
| 质粒大小: | 5522bp |
| 质粒标签: | His Tag (6x), C-Myc Epitope Tag |
| 原核抗性: | 氨苄青霉素Amp |
| 筛选标记: | 新霉素Neo |
| 克隆菌株: | DH5α |
| 培养条件: | 37℃，有氧，LB |
| 表达宿主: | 哺乳细胞 |
| 诱导方式: | IPTG或乳糖及其类似物 |
| 5'测序引物: | T7 Fwd:5d[TAATACGACTCACTATAGGG]3 |
| 3'测序引物: | 根据序列设计 |
质粒简介
pcDNA3.1/myc-His(-) A, B, and C are 5.5 kb vectors designed for overproduction of recombinant proteins in mammalian cell lines. The vectors are supplied in three reading frames to facilitate in frame cloning, with a C-terminal peptide containing a polyhistidine metal-binding tag and the myc (c-myc) epitope. The human cytomegalovirus immediate-early (CMV) promoter provides highlevel expression in a wide range of mammalian cells. In addition, the vector will replicate episomally in cell lines that are latently infected with SV40 or that express the SV40 large T antigen (e.g. COS7). High-level stable and nonreplicative transient expression can be carried out in most mammalian cells. The control plasmid, pcDNA3.1/myc-His(-)/lacZ, is the pcDNA3.1/myc-His(-) A vector with a 3.2 kb fragment containing the β-galactosidase gene cloned in frame with the C-terminal peptide. It is included for use as a positive control for transfection, expression, and detection in the cell line of choice.
质粒图谱
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载体pCDNA3.1(-)/Myc-His A
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