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- YJ-P179577
- 2025年07月10日
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- 文献和实验
- 技术资料
- 保存条件：
RT(常温）
- 保质期：
详见标签
- 库存：
100
- 供应商：
上海一基实业有限公司
- CAS号：
143028-99-3
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文献和实验96-well RNA In Situ Hybridization Protocol
). To stop development of individual wells, remove staining solution and add 200ul PBT. 26. To stop entire plate, rinse plate 3X with PBT. 27. Add 200ul 70% gycerol to each well. 28. Embryos are ready to mount or dissect once they have settled
Protocol for Trichloroacetic Acid (TCA) Precipitation
of radiolabeled nucleotide incorporation in a polymerization reaction by TCA precipitation. In the protocol, nucleic acid synthesis reactions are carried out in the presence of a radiolabeled nucleotide (e.g., 32 P-dATP or 32 P-UTP). Then, the synthesized polymers
The ribonuclease protection assay (RPA)
, the probe synthesis is performed during the afternoon Day 1, hybridizations are incubated overnight, and RNase treatments and gel electrophoresis are performed early on Day 2. Probe Synthesis: 1. Bring the [a-32P]UTP, GACU nucleotide pool, DTT
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