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- YJ-P197178
- 2025年07月13日
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863578-24-9
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文献和实验Preparation of probes for in situ hybridization
I. DNA oligonucleotide probes: DNA oligonucleotides are the preferable probes at present. They are synthesized chemically, which allows for the incorporation of amino-modified nucleotides (amino-allyl T) at defined positions
Preparation of probes for in situ hybridization
with ribosomal RNA (Witkiewicz H, Bolander ME, Edwards DR, 1993, Biotechniques vol 14, pp 458-463). III. Chemical conjugation of the amino-modified nucleic acids with activated fluorophores: The nucleic acid to be labelled should be resuspended
Synthesis and Probing of Membrane-bound Peptide Arrays
upon cleavage of the peptide. We have successfully added biotin via its in situ formed HOBt-ester (normal activation procedure) or fluorescein via its isothiocyanate (FITC) dissolved in DMF. After the final amino acid elongation cycle
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