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- 详细信息
- 文献和实验
- 技术资料
- 保存条件:
RT(常温)
- 保质期:
详见标签
- 库存:
100
- 供应商:
上海一基实业有限公司
- CAS号:
37691-11-5
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文献和实验Purification and Characterization of Crustacean Calpain-like Proteinases
and calpeptin), iodoacetate, iodoacetamide, Hg2+ , pCMB. and JV-ethylmaleimide (NEM) (1,2 ,7 ). The serine proteinase inhibitors tosyllysyl chloromethyl ketone (TLCK), tosylphenylalanine chloromethyl ketone (TPCK), leupeptin, and antipain are effective
GST fusion protein purification GST融合蛋白纯化
antipain " "check pH!GUSHISTOCHEMICAL STAINING1)determine number of slides needed,multiply by 0.75ml2)make up required vol of stain:for 10ml4℃ 5mg X-Gus50μl nn dimethyl formamide,dissolve10ml 50mM NaPO4 pH 73)sections best cut with vibrating knifefor
GST fusion protein purification--GST融合蛋白纯化
10 add before use0.5mM DTT 1M 0.5 "0.5mM PMSF 0.5mM 10 "5μg/ml Leupeptin 5mg/ml dilute before use5mg/ml antipain " "check pH!GUSHISTOCHEMICAL STAINING1)determine number of slides needed,multiply by 0.75ml2)make up required vol of stain:for 10ml4℃ 5mg
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