Organism SpeciesMus musculus (Mouse) Same name, Different species.
Sample Typeserum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Test MethodDouble-antibody Sandwich
Assay Length3h
Detection Range7.8-500pg/mL
SensitivityThe minimum detectable dose of this kit is typically less than 2.7pg/mL.
SpecificityThis assay has high sensitivity and excellent specificity for detection of Interleukin 12 (IL12). No significant cross-reactivity or interference between Interleukin 12 (IL12) and analogues was observed.
ApplicationsEnzyme-linked immunosorbent assay for Antigen Detection.
1. Prepare all reagents, samples and standards; 2. Add 100µL standard or sample to each well. Incubate 1 hours at 37°C; 3. Aspirate and add 100µL prepared Detection Reagent A. Incubate 1 hour at 37°C; 4. Aspirate and wash 3 times; 5. Add 100µL prepared Detection Reagent B. Incubate 30 minutes at 37°C; 6. Aspirate and wash 5 times; 7. Add 90µL Substrate Solution. Incubate 10-20 minutes at 37°C; 8. Add 50µL Stop Solution. Read at 450nm immediately.