迷你快速旋转核糖核酸柱

产品名称
ApplicationReady-to-use, disposable, microfuge-compatible columns designed for the removal of unincorporated radiolabeled nucleotides from RNA labeled by polymerase or end-labeling techniques.[1][2][3]
Use the highly purified RNA in northern blotting.
The mini Quick Spin columns are designed for use with variable-speed, fixed-angle microcentrifuges.Features and Benefits• Save time and effort with ready-to-use columns.
• Purify more nucleic acid in less time - process up to 75μl of sample in only 7 minutes.
• Minimize sample loss while maximizing yield - short and effective two-step procedure.
• Easy-to-use - column and cap allow for rapid resuspension of the gel matrix, and the bottom tip can be quickly snapped off.
• Ensure optimal performance and reproducibility with quality tested columns:
High recovery of radiolabeled RNA
Maximum retention of unincorporated nucleotides
Absence of RNases

Contents
Pre-packed, pre-swollen, microfuge-compatible columns with a cap and snap-off bottom tip.
Suspension of Sephadex G-50 in STE buffer (10mM Tris-HCI, pH 7.5, 1mM EDTA, 100mM NaCl).General description下载特定产品批次和规格的CofA、SDS和IFU。 Ready-to-use, microcentrifuge-compatible chromatography columns for quick and efficient purification of RNA from labeling reactions.Other Notes仅用于生命科学研究。不可用于诊断。PrincipleThe mini Quick Spin gel filtration columns separate molecules based on their relative size. This rapid chromatographic separation can be done in a conventional tabletop microcentrifuge. During centrifugation, mini Quick Spin Columns allow larger molecules (DNA, RNA, or oligonucleotides) to pass through quickly while retaining smaller molecules, such as unincorporated nucleotides.Preparation NoteSample size: 20 – 75μl
Time required: 7 minutes
Reactions/standard sample: 50QualityRNases are not detected, according to the current Quality Control procedures.SpecificationsRecovery of labeled RNA: ≥80%
Retention of unincorporated nucleotides: >99%