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- 详细信息
- 技术资料
- 保存条件:
-20摄氏度保存
- 保质期:
1年
- 英文名:
pSilencer 2.1-U6 hygro
- 库存:
大量
- 供应商:
上海沪震生物科技有限公司
- 规格:
盒
pSilencer 2.1-U6 hygro产品信息
订购信息
| 货号 | 载体名称 | 出品公司 | 载体用途 | 价格 | 到货周期 |
| HZ0338 | pSilencer 2.1-U6 hygro | RNAi载体 | 1000 | 现货 |
载体描述
This Ambion? vector is for the expression of siRNA. It has an antibiotic resistance gene (hygromycin) to enable selection of transfected cells and features the human RNA polymerase III promoter (U6). Sufficient reagents are provided for 20 reactions.
? Select transfected cells to enrich the population of cells expressing your siRNA
? Eliminate the need to synthesize RNA oligonucleotides for RNAi experiments
? Supplied linearized and ready for ligation
Compensate for Low Transfection Efficiencies and Perform Long-Term Studies
The use of mammalian siRNA expression vectors with antibiotic selectable markers conveys many benefits. Selectable markers can help compensate for poor plasmid transfection efficiencies seen with some cell lines. In these cases, only a fraction of the transfected cells express the siRNA, and reduction in target gene expression with even a potent siRNA can be difficult to detect. Use of a selectable marker and transient antibiotic selection permits.
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